Methyl-Lysine Antibody - Azide and BSA Free

Western Blot: Methyl-Lysine Antibody [NBP3-15955] - Analysis of extracts of various cell lines, using Methyl-Lysine antibody (NBP3-15955) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunoprecipitation: Methyl-Lysine Antibody [NBP3-15955] - Analysis of 1.5mg extracts of HeLa cells using 30ug Methyl-Lysine antibody (NBP3-15955). Western blot was performed from the immunoprecipitate using TriMethyl-Histone H3-K27 antibody (A2363) at a dilition of 1:1000.

Chromatin Immunoprecipitation: Methyl-Lysine Antibody - Azide and BSA Free [Methyl-Lysine] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Methyl-Lysine antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

• Reactivity: All-NA
• Applications: WB, ELISA, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Methyl-Lysine Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Methyl-Lysine Antibody - Azide and BSA Free (NBP3-15955) is a polyclonal antibody validated for use in WB, ELISA, IP and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to a sequence containing Tri-methylated K.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 3μg antibody for 5μg-10μg of Chromatin
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • Immunoprecipitation 5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • Western Blot 1:500 - 1:1000

Reactivity Notes

Wide Range Predicted

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for Methyl-Lysine Antibody - Azide and BSA Free

• Dimethylysine
• Methyl lysine
• methyllysine
• monomethyl lysine
• N epsilon dimethyl lysine
• N epsilon mono methyllysine
• N epsilon trimethyl lysine
• N(epsilon) trimethyl lysine
• Trimethyl lysine

Background

Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves methylation (1). Lysine can be methylated once, twice or three times by lysine methyltransferases. The transfer of methyl groups from S-adenosyl methionine to histones is catalyzed by enzymes known as histone methyltransferases. Histones which are methylated on certain residues can act epigenetically to repress or activate gene expression (1, 2). The transcriptional repressor SUV39H1 can encode novel enzymes which selectively methylate histone H3 at lysine 9. SUV39H1 places a methyl marker on histone H3, which is then recognized by HP1 through its chromo domain. This model may also explain the stable inheritance of the heterochromatic state (3). Some studies have also speculated a stimulatory role for transcription by methylated histone lyside 4 due to its presence at active transcription sites (4-6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov