metallothionein Antibody (N11-G)

Western Blot: metallothionein Antibody (N11-G) [NBP1-79083] - Rabbit liver. Lane 1 - 1ug; Lane 2 - 3ug; Lane 3 - 5ug of protein loaded.

• Reactivity: Hu, Rb
• Applications: WB, ELISA
• Clone: N11-G
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

metallothionein Antibody (N11-G) Summary

• Description: This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
• Immunogen: Peptide corresponding to the N-terminal sequence of human metallothionein (covering isoforms 1A, B, E, F, G, H, I, L, M, Q, S, Y, 2A)
• Specificity: NBP1-79083 immunogen is identical and covers isoforms#: 1A P04731, #1B P07438, #1E P04732 ,#1F P04733, #1G P13640, #1H P80294, #1I P80295, #1L Q93083, #1M Q8N339, #1Q Q86YX0, #1S Q86YX5, #1Y Q8TDC4, #2A P18055.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Western Blot 1:1000
• Application Notes: For western blots, incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature.

Reactivity Notes

Human - isoforms: 1A, 1B, 1E, 1F, 1G,1H, 1I,1L, Rabbit - isoforms: 1A, 2A

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Background

Metallothioneins (MTs) are a class of ubiquitous, low molecular weight, cysteine-rich, metal binding proteins that function in the metabolism of zinc and copper. MT protects against exposure to toxic metals such as mercury and cadmium as well as a variety of other stress conditions. There are four mammalian MT isoforms: MT-I, -II, -III, -IV, each of which is encoded by individual genes. MT expression is induced by heavy metal cations, starvation, elevated temperature, lipopolysaccharide, inflammatory cytokines, free radicals, and ionizing radiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.