Luciferase Antibody (LUC-Y)

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Product Details

Summary
Reactivity PpSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-Fr
Clone
LUC-Y
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Luciferase Antibody (LUC-Y) Summary

Immunogen
Recombinant luciferase expressed in E. coli.
Specificity
Detects the expression of the luciferase gene of the North American firefly Photinus pyralis in cells and tissues.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
For Flow Cytometry, membrane permeabilisation is required. For Flow Cytometry we recommend using 0.1ug per 1 x 10^6 cells.

Reactivity Notes

Reacts with Insect.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Luciferase Antibody (LUC-Y)

  • ec 1.13.12.7
  • Fluc
  • Luciferase
  • luciferin 4 monooxygenase

Background

Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg+2 and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Luciferase Antibody (NB100-64800) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Luciferase Products

Research Areas for Luciferase Antibody (NB100-64800)

Find related products by research area.

Blogs on Luciferase.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

Luciferase: Shining a Light to See Inside Living Animal Models
The luciferase reporter is a valuable tool for research into physiology and disease. Light emitted from luciferase enables the monitoring of xenografted tumors, specific cell types, gene expression and pathogens within live animals over time using bio...  Read full blog post.

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