LRRC45 Antibody

Western Blot: LRRC45 Antibody [NBP1-93508] - Analysis in control (vector only transfected HEK293T lysate) and LRRC45 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: LRRC45 Antibody [NBP1-93508] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & centrosome.

Immunohistochemistry-Paraffin: LRRC45 Antibody [NBP1-93508] - Staining of human lymph node.

Immunohistochemistry-Paraffin: LRRC45 Antibody [NBP1-93508] - Staining of human fallopian tube shows high expression.

Immunohistochemistry-Paraffin: LRRC45 Antibody [NBP1-93508] - Staining of human skeletal muscle shows low expression as expected.

Immunohistochemistry-Paraffin: LRRC45 Antibody [NBP1-93508] - Staining in human fallopian tube and skeletal muscle tissues using anti-LRRC45 antibody. Corresponding LRRC45 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: LRRC45 Antibody [NBP1-93508] - Staining of human fallopian tube, lymph node, placenta and skeletal muscle using Anti-LRRC45 antibody NBP1-93508 (A) shows similar protein distribution across tissues to independent antibody NBP1-93509 (B).

Immunohistochemistry-Paraffin: LRRC45 Antibody [NBP1-93508] - Staining of human placenta.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

LRRC45 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: RHSIINALKAKLQMTEAALALSEQKAQDLGELLATAEQEQLSLSQRQAKELKLEQQEAAERESKLLRDLSAANEKNLLLQNQVDELERKFRCQQE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LRRC45
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  LRRC45 Protein (NBP1-93508PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (87%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for LRRC45 Antibody

• leucine rich repeat containing 45
• leucine-rich repeat-containing protein 45
• MGC20806

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LRRC45