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Lambda Light Chain Antibody (4C2) (NBP1-45071)

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Flow Cytometry: Lambda Light Chain Antibody (4C2) [NBP1-45071] - Surface staining of human peripheral blood with anti-human lambda light chain (4C2) purified, GAM-APC.
Flow Cytometry: Lambda Light Chain Antibody (4C2) [NBP1-45071] - Surface staining of human peripheral blood with anti-human lambda light chain (4C2) purified, GAM-APC.
Flow Cytometry: Lambda Light Chain Antibody (4C2) [NBP1-45071] - B lymphocytes (CD19+) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain detected by A8B5 antibody and lambda light chain by 4C2 antibody, CD19 by ...read more
Flow Cytometry: Lambda Light Chain Antibody (4C2) [NBP1-45071] - Surface staining of human peripheral blood with anti-human lambda light chain (4C2) FITC.
Flow Cytometry: Lambda Light Chain Antibody (4C2) [NBP1-45071] - Surface staining of human peripheral blood with anti-human lambda light chain (4C2) APC.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, IHC, IHC-Fr, IHC-P, CyTOF-ready
Clone
4C2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP1-45071AF405
Alexa Fluor 488
NBP1-45071AF488
Alexa Fluor 647
NBP1-45071AF647
Alexa Fluor 700
NBP1-45071AF700
Allophycocyanin
NBP1-45071APC
DyLight 350
NBP1-45071UV
DyLight 405
NBP1-45071V
DyLight 488
NBP1-45071G
DyLight 680
NBP1-45071FR
DyLight 755
NBP1-45071IR
FITC
NBP1-45069
PE
NBP1-45071PE
PerCP
NBP1-45071PCP
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Lambda Light Chain Antibody (4C2) Summary

Immunogen
The antibody 4C2 reacts with lambda light chains (22.5 kDa) of human immunoglobulin.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IGLC2
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:2000
  • Flow Cytometry 2 - 8 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:200
  • CyTOF-ready

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Lambda Light Chain Antibody (4C2)

  • IGLC
  • immunoglobulin lambda constant group

Background

Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: BCR Antibody [NBP1-19097] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-BCR antibody used for IP at 10 mcg/mg lysate. BCR was also immunoprecipitated by rabbit anti-BCR antibody NBP1-19098, which recognizes a downstream epitope
Immunocytochemistry/Immunofluorescence: BCR Antibody [NBP1-19097] - Human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene). Antibody used at a dilution of 1:1,000 (1ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG - AlexaFluor 488 used at a dilution of 1:1,000.
Flow Cytometry: BCR Antibody [NBP1-19097] - Flow Cytometric Detection of BCR. 1 x 106 K562 cells were fixed, permeabilized, and stained with 0.25 mcg anti-BCR in a 150 mcl reaction. Isotype anti-KLH control (black), no antibody (red) and anti-BCR (blue).
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, PLA
Lambda Light Chain
Antibody (ICO106)
NBP2-29462
Immunohistochemistry-Paraffin: Lambda light chain Antibody (ICO106) [NBP2-29462] - Staining of non-Hodgkin's lymphoma. Note cell membrane and cytoplasmic staining.
Immunohistochemistry-Paraffin: Lambda light chain Antibody (ICO106) [NBP2-29462] - Formalin-fixed, paraffin-embedded non-Hodgkin's lymphoma stained with Lambda antibody (1:200), peroxidase-conjugate and DAB chromogen. Note the cell membrane and cytoplasmic staining.
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Myoglobin Antibody
(5A2G8)
NB110-89456
Western Blot: Myoglobin Antibody (5A2G8) [NB110-89456] - Analysis using Myoglobin mouse mAb against truncated Myoglobin recombinant protein(AA: 2-154).
Immunohistochemistry-Paraffin: Myoglobin Antibody (5A2G8) [NB110-89456] - Analysis of paraffin-embedded human skeletal muscle tissue showing cytoplasmic localization using anti-Myoglobin antibody with DAB staining.
Species: Hu
Applications: WB, ELISA, IHC, IHC-Fr, IHC-P
TCIRG1 Antibody
NBP1-89333
Immunocytochemistry/Immunofluorescence: TCIRG1 Antibody [NBP1-89333] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunohistochemistry-Paraffin: TCIRG1 Antibody [NBP1-89333] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
(1 Publication)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
CRAT Antibody
NBP1-86616
Western Blot: CRAT Antibody [NBP1-86616] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: CRAT Antibody [NBP1-86616] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Species: Hu
Applications: WB, IHC, IHC-P
GLYAT Antibody
(4D1)
NBP2-45897
Western Blot: GLYAT Antibody (4D1) [NBP2-45897] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLYAT.
Immunohistochemistry: GLYAT Antibody (4D1) [NBP2-45897] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: GLYAT Antibody (4D1) [NBP2-45897] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Species: Hu
Applications: WB, IHC
Kappa Light Chain
Antibody (L1C1)
NBP2-15191
Western Blot: Kappa Light Chain Antibody (L1C1) [NBP2-15191] - Analysis of Raji cell Lysate using kappa light chain monoclonal Antibody (L1C1).
Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (L1C1) [NBP2-15191] - Formalin-paraffin human tonsil stained with Kappa Light Chain Antibody (L1C1) Note cell membrane & cytoplasmic staining.
Simple Western: Kappa Light Chain Antibody (L1C1) [NBP2-15191] - Simple Western lane view shows a specific band for Kappa Light Chain in 0.2 mg/ml of h serum, h tonsil lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Species: Hu
Applications: WB, Simple Western, Flow, IHC-Fr, IHC-P
Catalase Antibody
NBP2-24916
Western Blot: Catalase Antibody [NBP2-24916] - Analysis in liver lysate of 1) human 2) mouse and 3) rat at 0.1 ug/ml.
Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-24916] - Staining of human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
(1 Review)
(4 Publications)
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
14-3-3 eta
Antibody (3G12)
NBP1-92691
Western Blot: 14-3-3 eta Antibody (3G12) [NBP1-92691] - Crude HeLa cell homogenate blotted with NBP1-92691 (left lane) and various molecular weight standards (right lane - numbers indicate apparent SDS-PAGE molecular weight in kDa). The 14-3-3 eta (3G12) monoclonal binds strongly and cleanly to a band at about 28kDa.
Immunocytochemistry/Immunofluorescence: 14-3-3 eta Antibody (3G12) [NBP1-92691] - Rat mixed neuron/glial cultures stained with NBP1-92691 antibody (red). Neuronal perikarya are very rich in 14.3.3 eta which has a diffuse cytoplasmic staining pattern. Blue is a DNA stain.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
RFC2 Antibody (2B2)
NBP2-45570
Western Blot: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2.
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Flow Cytometry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by RFC2 antibody.
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Applications: WB, Flow, IHC
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Antibody
NBP2-30949
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RFC1 Antibody
NB100-230
Western Blot: RFC1 Antibody [NB100-230] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFC1 antibody (lot was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: RFC1 Antibody [NB100-230] - Samples: Whole cell lysate (50, 15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFC1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Proximity Ligation Assay: RFC1 Antibody [NB100-230] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC1 (NB100-230) and rabbit anti-human BLM (NB100-214). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Species: Hu
Applications: WB, IP, PLA
alpha-Sarcoglycan
Antibody
NBP1-90306
Western Blot: alpha Sarcoglycan Antibody [NBP1-90306] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
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(1 Publication)
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CD37 Antibody
NBP2-33969
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Species: Hu
Applications: WB, IHC, IHC-P
RPLP1 Antibody
NBP1-81293
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Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
c-Abl Antibody
[Unconjugated]
AF5414
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Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for c‑Abl at approximately 149 kDa (as indicated) using 10 µg/mL of Goat Anti-Human c‑Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
(1 Publication)
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RAB36 Antibody
(6A6)
H00009609-M01
Western Blot: RAB36 Antibody (6A6) [H00009609-M01] - RAB36 monoclonal antibody (M01), clone 6A6 Analysis of RAB36 expression in A-431.
Immunocytochemistry/Immunofluorescence: RAB36 Antibody (6A6) [H00009609-M01] - Analysis of monoclonal antibody to RAB36 on A-431 cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: RAB36 Antibody (6A6) [H00009609-M01] - Analysis of monoclonal antibody to RAB36 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1.5 ug/ml.
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Lambda Light Chain
Antibody (4C2)
NBP1-45071
Lambda Light Chain Antibody (4C2)
Species: Hu
Applications: ELISA, Flow, IHC, IHC-Fr, IHC-P, CyTOF-ready

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-45071AF405
Alexa Fluor 488 Labeled NBP1-45071AF488
Alexa Fluor 647 Labeled NBP1-45071AF647
Alexa Fluor 700 Labeled NBP1-45071AF700
Allophycocyanin Labeled NBP1-45071APC
DyLight 350 Labeled NBP1-45071UV
DyLight 405 Labeled NBP1-45071V
DyLight 488 Labeled NBP1-45071G
DyLight 680 Labeled NBP1-45071FR
DyLight 755 Labeled NBP1-45071IR
FITC Labeled NBP1-45069
PE Labeled NBP1-45071PE
PerCP Labeled NBP1-45071PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol IGLC2

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