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Lambda Light Chain Antibody (4C2)

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Flow Cytometry: Lambda Light Chain Antibody (4C2) [NBP1-45071] - Surface staining of human peripheral blood with anti-human lambda light chain (4C2) purified, GAM-APC.
Flow Cytometry: Lambda Light Chain Antibody (4C2) [NBP1-45071] - Surface staining of human peripheral blood with anti-human lambda light chain (4C2) purified, GAM-APC.
Flow Cytometry: Lambda Light Chain Antibody (4C2) [NBP1-45071] - B lymphocytes (CD19+) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain detected by A8B5 antibody and lambda light chain by 4C2 antibody, CD19 by ...read more
Flow Cytometry: Lambda Light Chain Antibody (4C2) [NBP1-45071] - Surface staining of human peripheral blood with anti-human lambda light chain (4C2) FITC.
Flow Cytometry: Lambda Light Chain Antibody (4C2) [NBP1-45071] - Surface staining of human peripheral blood with anti-human lambda light chain (4C2) APC.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, IHC, IHC-Fr, IHC-P, CyTOF-ready
Clone
4C2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP1-45071AF405
Alexa Fluor 488
NBP1-45071AF488
Alexa Fluor 647
NBP1-45071AF647
Alexa Fluor 700
NBP1-45071AF700
Allophycocyanin
NBP1-45071APC
DyLight 350
NBP1-45071UV
DyLight 405
NBP1-45071V
DyLight 488
NBP1-45071G
DyLight 680
NBP1-45071FR
DyLight 755
NBP1-45071IR
FITC
NBP1-45069
Janelia Fluor 549
NBP1-45071JF549
Janelia Fluor 646
NBP1-45071JF646
PE
NBP1-45071PE
PerCP
NBP1-45071PCP
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Lambda Light Chain Antibody (4C2) Summary

Immunogen
The antibody 4C2 reacts with lambda light chains (22.5 kDa) of human immunoglobulin.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IGLC2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA 1:2000
  • Flow Cytometry 2 - 8 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:200
  • CyTOF-ready
Theoretical MW
22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Lambda Light Chain Antibody (4C2)

  • IGLC
  • immunoglobulin lambda constant group

Background

Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: BCR Antibody [NBP1-19097] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-BCR antibody used for IP at 10 mcg/mg lysate. BCR was also immunoprecipitated by rabbit anti-BCR antibody NBP1-19098, which recognizes a downstream epitope
Immunocytochemistry/Immunofluorescence: BCR Antibody [NBP1-19097] - Human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene). Antibody used at a dilution of 1:1,000 (1ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG - AlexaFluor 488 used at a dilution of 1:1,000.
Flow Cytometry: BCR Antibody [NBP1-19097] - Flow Cytometric Detection of BCR. 1 x 106 K562 cells were fixed, permeabilized, and stained with 0.25 mcg anti-BCR in a 150 mcl reaction. Isotype anti-KLH control (black), no antibody (red) and anti-BCR (blue).
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, PLA
Lambda Light Chain
Antibody (ICO106)
NBP2-29462
Immunohistochemistry-Paraffin: Lambda Light Chain Antibody (ICO106) [NBP2-29462] - Staining of non-Hodgkin's lymphoma. Note cell membrane and cytoplasmic staining.
Immunohistochemistry-Paraffin: Lambda Light Chain Antibody (ICO106) [NBP2-29462] - Formalin-fixed, paraffin-embedded non-Hodgkin's lymphoma stained with Lambda antibody (1:200), peroxidase-conjugate and DAB chromogen. Note the cell membrane and cytoplasmic staining.
SDS-Page: Lambda Light Chain Antibody (ICO106) [NBP2-29462]
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Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PAGE
Myoglobin Antibody
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NBP1-47364
Western Blot: Myoglobin Antibody (6H8B5) [NBP1-47364] - Analysis using Myoglobin mouse mAb against truncated Myoglobin recombinant protein(AA: 2-154).
Immunohistochemistry-Paraffin: Myoglobin Antibody (6H8B5) [NBP1-47364] - Analysis of human skeletal muscle tissue showing cytoplasmic localization using anti-Myoglobin antibody with DAB staining.
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Applications: WB, ELISA, IHC, IHC-Fr, IHC-P
TCIRG1 Antibody
NBP1-89333
Immunocytochemistry/Immunofluorescence: TCIRG1 Antibody [NBP1-89333] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TCIRG1 Antibody [NBP1-89333] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
(1 Publication)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
CRAT Antibody
NBP1-86615
Western Blot: CRAT Antibody [NBP1-86615] - Staining in human testis and tonsil tissues using anti-CRAT antibody. Corresponding CRAT RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CRAT Antibody [NBP1-86615] - Staining of human tonsil shows low expression as expected.
Western Blot: CRAT Antibody [NBP1-86615] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Species: Hu
Applications: WB, IHC, IHC-P
PDGF-BB
[Unconjugated]
220-BB
1 μg/lane of Recombinant Human PDGF-BB was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 13 kDa and 28 kDa, respectively.
Recombinant Human PDGF-BB (Catalog # 220-BB) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner. The ED50 for this effect is 1.5-6 ng/mL.
(5 Reviews)
(91 Publications)
Species: Hu
GLYAT Antibody
NBP2-55298
Western Blot: GLYAT Antibody [NBP2-55298] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Immunohistochemistry-Paraffin: GLYAT Antibody [NBP2-55298] - Staining in human kidney and skeletal muscle tissues using anti-GLYAT antibody. Corresponding GLYAT RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: GLYAT Antibody [NBP2-55298] - Staining of human kidney shows high expression.
Species: Hu
Applications: WB, IHC, IHC-P
Kappa Light Chain
Antibody (L1C1)
NBP2-15191
Western Blot: Kappa Light Chain Antibody (L1C1) [NBP2-15191] - Analysis of Raji cell Lysate using kappa light chain monoclonal Antibody (L1C1).
Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (L1C1) [NBP2-15191] - Formalin-paraffin human tonsil stained with Kappa Light Chain Antibody (L1C1) Note cell membrane & cytoplasmic staining.
Simple Western: Kappa Light Chain Antibody (L1C1) [NBP2-15191] - Simple Western lane view shows a specific band for Kappa Light Chain in 0.2 mg/ml of h serum, h tonsil lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Species: Hu
Applications: WB, Simple Western, Flow, IHC-Fr, IHC-P, PAGE
Catalase Antibody
NBP2-24916
Western Blot: Catalase Antibody [NBP2-24916] - Analysis in liver lysate of 1) human 2) mouse and 3) rat at 0.1 ug/ml.
Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-24916] - Staining of human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
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14-3-3 eta
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NBP1-92691
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RFC2 Antibody
(OTI2B2)
NBP2-45570
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Flow Cytometry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by RFC2 antibody.
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Protamine 1
Antibody
NBP2-30949
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RFC1 Antibody
NB100-230
Western Blot: RFC1 Antibody [NB100-230] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFC1 antibody (lot was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: RFC1 Antibody [NB100-230] - Samples: Whole cell lysate (50, 15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFC1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Proximity Ligation Assay: RFC1 Antibody [NB100-230] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC1 (NB100-230) and rabbit anti-human BLM (NB100-214). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
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alpha-Sarcoglycan
Antibody
NBP1-90306
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CD37 Antibody (2B8)
NBP1-28869
Immunocytochemistry: CD37 Antibody (2B8) [NBP1-28869] - Confocal immunofluorescence analysis of methanol-fixed BCBL-1 (left) and L1210 (right) cells using CD37 mouse mAb(green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
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Immunocytochemistry/Immunofluorescence: RPLP1 Antibody [NBP1-81293] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RPLP1 Antibody [NBP1-81293] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
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c-Abl Antibody
[Unconjugated]
AF5414
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF‑7 human breast cancer cell line, and MDA‑MB‑453 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human c-Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by HRP-conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for c-Abl at approximately 135 - 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for c‑Abl at approximately 149 kDa (as indicated) using 10 µg/mL of Goat Anti-Human c‑Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
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Applications: WB, Simple Western
RAB36 Antibody
(6A6)
H00009609-M01
Western Blot: RAB36 Antibody (6A6) [H00009609-M01] - RAB36 monoclonal antibody (M01), clone 6A6 Analysis of RAB36 expression in A-431.
Immunocytochemistry/Immunofluorescence: RAB36 Antibody (6A6) [H00009609-M01] - Analysis of monoclonal antibody to RAB36 on A-431 cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: RAB36 Antibody (6A6) [H00009609-M01] - Analysis of monoclonal antibody to RAB36 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1.5 ug/ml.
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-45071AF405
Alexa Fluor 488 Labeled NBP1-45071AF488
Alexa Fluor 647 Labeled NBP1-45071AF647
Alexa Fluor 700 Labeled NBP1-45071AF700
Allophycocyanin Labeled NBP1-45071APC
DyLight 350 Labeled NBP1-45071UV
DyLight 405 Labeled NBP1-45071V
DyLight 488 Labeled NBP1-45071G
DyLight 680 Labeled NBP1-45071FR
DyLight 755 Labeled NBP1-45071IR
FITC Labeled NBP1-45069
PE Labeled NBP1-45071PE
PerCP Labeled NBP1-45071PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol IGLC2