Lambda Light Chain Antibody (4C2) [FITC]

• Reactivity: Hu
• Applications: ELISA, Flow, IHC
• Clone: 4C2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

Lambda Light Chain Antibody (4C2) [FITC] Summary

• Description: Store in the dark. Avoid prolonged exposure to light.
• Immunogen: The antibody 4C2 reacts with lambda light chains (22.5 kDa) of human immunoglobulin.
• Specificity: The mouse monoclonal antibody H4B4 recognizes CD107b / LAMP-2, an extensively glycosylated 100-120 kDa widely expressed lysosome-associated protein.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGLC1
• Purity: Size Exclusion Chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:200
• Theoretical MW: 22.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS and 0.2% (w/v) high grade BSA (protease free)
• Preservative: 0.09% Sodium Azide
• Purity: Size Exclusion Chromatography

Alternate Names for Lambda Light Chain Antibody (4C2) [FITC]

• IGLC
• immunoglobulin lambda constant group

Background

All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain are present in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGLC1