![Western Blot: Lactyl-Lysine Antibody [NBP3-15602] - analysis of MCF7, using Pan Lactic acid-Lysine Rabbit pAb at 1:400 dilution.MCF7 cells were treated by Sodium lactate(100mM) for 24h.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/antibody/nbp3-15602_rabbit-lactyl-lysine-pab-western-blot-91120239360..jpg "Western Blot: Lactyl-Lysine Antibody [NBP3-15602] - analysis of MCF7, using Pan Lactic acid-Lysine Rabbit pAb at 1:400 dilution.MCF7 cells were treated by Sodium lactate(100mM) for 24h.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 60s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit Lactyl-Lysine Antibody - BSA Free (NBP3-15602) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | A synthetic peptide corresponding to a sequence containing crotonylated K. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Reviewed Applications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol |
| Preservative | 0.09% Sodium Azide |
| Purity | Affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Simin Yan |
WB | Human and human pulmonary arterial smooth muscle cells | 10/13/2025 |
Summary
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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| Simin Yan 10/13/2025 |
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| Application: | WB | |
| Species: | Human and human pulmonary arterial smooth muscle cells |
![Western Blot: Lactyl-Lysine Antibody [NBP3-15602] - Analysis of NIH/3T3, using Pan Lactic acid-Lysine Rabbit pAb at 1:400 dilution.NIH/3T3 cells were treated by Sodium lactate(100mM) for 24h.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit Exposure time: 60s.](http://images.novusbio.com/fullsize/antibody/nbp3-15602_rabbit-lactyl-lysine-pab-western-blot-911202393713..jpg "Western Blot: Lactyl-Lysine Antibody [NBP3-15602] - Analysis of NIH/3T3, using Pan Lactic acid-Lysine Rabbit pAb at 1:400 dilution.NIH/3T3 cells were treated by Sodium lactate(100mM) for 24h.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit Exposure time: 60s.")
![Western Blot: Lactyl-Lysine Antibody [NBP3-15602] - Analysis of C6, using Pan Lactic acid-Lysine Rabbit pAb at 1:400 dilution.C6 cells were treated by Sodium lactate(100mM) for 24h.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/antibody/nbp3-15602_rabbit-lactyl-lysine-pab-western-blot-911202393834..jpg "Western Blot: Lactyl-Lysine Antibody [NBP3-15602] - Analysis of C6, using Pan Lactic acid-Lysine Rabbit pAb at 1:400 dilution.C6 cells were treated by Sodium lactate(100mM) for 24h.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 60s.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
