Insulin R/IGF-I R Heterotetramer [p Tyr1136, p Tyr1163, p Tyr1135, p Tyr1162] Antibody - Cross-reactive

Images

 
Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human ...read more
Insulin R phosphorylated at Y1162/1163 and IGF‑I R phsophorylated at Y1135/1136 were detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with ...read more
HeLa human cervical epithelial carcinoma cell line was unstimulated (light orange open histogram) or treated with 100 μM pervanadate for 15 minutes, then stained with Rabbit Anti-Human Phospho-Insulin R ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, CyTOF-ready, ICC, ICFlow
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Insulin R/IGF-I R Heterotetramer [p Tyr1136, p Tyr1163, p Tyr1135, p Tyr1162] Antibody - Cross-reactive Summary

Description
Antibody detects Insulin R [p Tyr1162, p Tyr1163] / IGF-I R phosphorylated [p Tyr1135, p Tyr1136]
Immunogen
Phosphopeptide containing human Insulin R Y1162/Y1163 sites
Modification
p Tyr1136, p Tyr1163, p Tyr1135, p Tyr1162
Specificity
Detects human Insulin R dually phosphorylated at Y1162 and Y1163, and human IGF-I R dually phosphorylated at Y1135 and Y1136.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Publications
Read Publications using
AF2507 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Insulin R/IGF-I R Heterotetramer

  • Insulin R/IGF-I R Heterotetramer

Background

The heterotetrameric receptors for insulin (INS R) and IGF-I (IGF-I R) are receptor tyrosine kinases that consist of two ligand-binding alpha subunits and two beta subunits. Ligand binding induces autophosphorylation on multiple tyrosine residues of beta  subunits. Phosphorylation of Tyr1162 and 1163 on INS R and Tyr1135 and 1136 on IGF‑I R stimulates intrinsic kinase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Insulin R/IGF-I R Heterotetramer Antibody (AF2507)(3)

Reviews for Insulin R/IGF-I R Heterotetramer Antibody (AF2507) (0)

There are no reviews for Insulin R/IGF-I R Heterotetramer Antibody (AF2507). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Insulin R/IGF-I R Heterotetramer Antibody (AF2507) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Insulin R/IGF-I R Heterotetramer Products

Insulin R/IGF-I R Heterotetramer AF2507

Blogs on Insulin R/IGF-I R Heterotetramer

There are no specific blogs for Insulin R/IGF-I R Heterotetramer, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Insulin R/IGF-I R Heterotetramer [p Tyr1136, p Tyr1163, p Tyr1135, p Tyr1162] Antibody - Cross-reactive and receive a gift card or discount.