Influenza B nucleoprotein Antibody - (B/Taiwan/753/2005) - BSA Free

Western Blot: Influenza B nucleoprotein Antibody [NBP3-12881] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza B nucleoprotein antibody (NBP3-12881) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Influenza B nucleoprotein Antibody [NBP3-12881] - Influenza B nucleoprotein antibody detects Influenza B nucleoproteinprotein by immunofluorescent analysis. Sample: Influenza B infected MDCK cells were fixed in paraformaldehyde. Green: Influenza B nucleoprotein stained by Influenza B nucleoprotein antibody (NBP3-12881) diluted at 1:2000. Blue: Hoechst 33342 staining.

Western Blot: Influenza B nucleoprotein Antibody [NBP3-12881] - Influenza B nucleoprotein antibody detects Influenza B nucleoproteinprotein by Western blot analysis. A. 5ul non-infected medium B. 5ul Influenza B (Taiwan B70555) infected medium 7.5 % SDS-PAGE Influenza B nucleoprotein antibody (NBP3-12881) dilution: 1:10000

Immunocytochemistry/Immunofluorescence: Influenza B nucleoprotein Antibody [NBP3-12881] - Influenza B nucleoprotein antibody detects Influenza B Virus nucleoprotein by immunofluorescent analysis. Sample: MDCK cells infected with Influenza B (Taiwan B70555). Green: Influenza B nucleoprotein stained by Influenza B nucleoprotein antibody (NBP3-12881) diluted at 1:500. Blue: Hoechst 33342 staining.

Sandwich ELISA: Influenza B nucleoprotein Antibody [NBP3-12881] - Sandwich ELISA detection of recombinant full-length Influenza B nucleoprotein, Flag tag using Influenza B nucleoprotein antibody [GT382] (NBP3-13500) as capture antibody at concentration of 5 ug/mL and Influenza B nucleoprotein antibody (NBP3-12881) as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.

Sandwich ELISA: Influenza B nucleoprotein Antibody [NBP3-12881] - Sandwich ELISA detection of recombinant full-length Influenza B nucleoprotein, Flag tag using Influenza B nucleoprotein antibody [GT371] (NBP3-13499) as capture antibody at concentration of 5 ug/mL and Influenza B nucleoprotein antibody (NBP3-12881) as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.

Sandwich ELISA: Influenza B nucleoprotein Antibody [NBP3-12881] - Sandwich ELISA detection of non-transfected and Influenza B nucleoprotein (B/Sydney/3/2004) transfected 293T whole cell extracts using Influenza B nucleoprotein antibody [GT382] (NBP3-13500) as capture antibody at concentration of 5 ug/mL and Influenza B nucleoprotein antibody (NBP3-12881) as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.

Sandwich ELISA: Influenza B nucleoprotein Antibody [NBP3-12881] - Sandwich ELISA detection of non-transfected and Influenza B nucleoprotein (B/Sydney/3/2004) nucleoprotein transfected 293T whole cell extracts using Influenza B nucleoprotein antibody [GT371] (NBP3-13499) as capture antibody at concentration of 5 ug/mL and Influenza B nucleoprotein antibody (NBP3-12881) as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.

• Reactivity: V-Vi
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Influenza B nucleoprotein Antibody - (B/Taiwan/753/2005) - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of Influenza B nucleoprotein (B/Taiwan/753/2005). The exact sequence is proprietary.
• Specificity: Influenza B Nucleoprotein (B/Taiwan/753/2005)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot

Reactivity Notes

Influenza B virus

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Influenza B nucleoprotein Antibody - (B/Taiwan/753/2005) - BSA Free

• Influenza B Virus NP
• NP
• Nucleoprotein

Background

Influenza virus B is a genus in the virus family Orthomyxoviridae. A limited host range means that Influenza virus B pandemics are rare. The nucleoprotein protects the negative strand viral RNA from nucleases by encapsidating it. encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Influenza B nucleoprotein Antibody (NBP3-12881). (Showing 1 - 1 of 1 FAQ).

1. We ordered your Influenza A nucleoprotein. We tested it. And it worked well for us. I am wondering if you also have Influenza B nucleoprotein, Recombinant. I don’t see it in your catalog.
   • At this time, it appears we only carry antibodies direct towards Influenza B nucleoprotein. We do not currently have a recombinant protein to this target. I apologize for the inconvenience.