Influenza A nucleoprotein Antibody (010) [Alexa Fluor® 532]

• Reactivity: V
• Applications: WB, ELISA
• Clone: 010
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 532

Influenza A nucleoprotein Antibody (010) [Alexa Fluor® 532] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Influenza A H3N2 (Uniprot#: P22435.2; Met1-Asn498).
• Specificity: Widely recognize Influenza A Nucleoprotein
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Pan Influenza A Has cross-reactivity in ELISA with: H1N1 (A/Brevig Mission/1/1918) NP H1N1 (A/Brisbane/02/2018) NP H1N1 (A/California/07/2009) NP H1N1 (A/Guangdong-Maonan/SWL1536/2019) NP H1N1 (A/Hawaii/70/2019) NP H1N1 (A/Michigan/45/2015 NP H1N1 (A/Puerto Rico/8/34/Mount Sinai) NP (I116M) H1N1 (A/Victoria/2570/2019)/(A/Wisconsin/588/2019) NP H2N2 (A/Ann Arbor/6/1960) NP H3N2 (A/Aichi/2/1968) NP H3N2 (A/Cambodia/e0826360/2020 (H3N2)-like NP H3N2 (A/Hong Kong/1/1968) NP H3N2 (A/Hong Kong/2671/2019) NP H3N2 (A/Hong Kong/45/2019) NP H3N2 (A/Hong Kong/4801/2014) NP H3N2 (A/Kansas/14/2017) NP H3N2 (A/Switzerland/9715293/2013) NP H7N9 (A/Anhui/1-BALF_RG6/2013) NP H7N9 (A/Shanghai/2/2013) NP Influenza B (B/Phuket/3073/2013) NP Influenza B (B/Brisbane/60/2008) NP Influenza B (B/Colorado/06/2017) NP No cross-reactivity in ELISA with: Influenza B (B/Florida/4/2006) NP Influenza B (B/Washington/02/2019) NP SARS-CoV-2 Nucleocapsid Protein HCoV-229E Nucleocapsid Protein HCoV-NL63 Nucleocapsid Protein HCoV-HKU1 Nucleocapsid Protein HCoV-OC43 Nucleocapsid Protein

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Influenza A nucleoprotein Antibody (010) [Alexa Fluor® 532]

• Influenza A virus NP
• NP
• NP-A
• Nucleocapsid protein
• Nucleoprotein

Background

The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.