Influenza A nucleoprotein Antibody (010) [Alexa Fluor™ Plus 594]

• Reactivity: V
• Applications: WB, ELISA
• Clone: 010
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 594

Influenza A nucleoprotein Antibody (010) [Alexa Fluor™ Plus 594] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Influenza A H3N2 (Uniprot#: P22435.2; Met1-Asn498).
• Specificity: Widely recognize Influenza A Nucleoprotein
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Pan Influenza A

Has cross-reactivity in ELISA with:
H1N1 (A/Brevig Mission/1/1918) NP
H1N1 (A/Brisbane/02/2018) NP
H1N1 (A/California/07/2009) NP
H1N1 (A/Guangdong-Maonan/SWL1536/2019) NP
H1N1 (A/Hawaii/70/2019) NP
H1N1 (A/Michigan/45/2015 NP
H1N1 (A/Puerto Rico/8/34/Mount Sinai) NP (I116M)
H1N1 (A/Victoria/2570/2019)/(A/Wisconsin/588/2019) NP
H2N2 (A/Ann Arbor/6/1960) NP
H3N2 (A/Aichi/2/1968) NP
H3N2 (A/Cambodia/e0826360/2020 (H3N2)-like NP
H3N2 (A/Hong Kong/1/1968) NP
H3N2 (A/Hong Kong/2671/2019) NP
H3N2 (A/Hong Kong/45/2019) NP
H3N2 (A/Hong Kong/4801/2014) NP
H3N2 (A/Kansas/14/2017) NP
H3N2 (A/Switzerland/9715293/2013) NP
H7N9 (A/Anhui/1-BALF_RG6/2013) NP
H7N9 (A/Shanghai/2/2013) NP
Influenza B (B/Phuket/3073/2013) NP
Influenza B (B/Brisbane/60/2008) NP
Influenza B (B/Colorado/06/2017) NP

No cross-reactivity in ELISA with:
Influenza B (B/Florida/4/2006) NP
Influenza B (B/Washington/02/2019) NP
SARS-CoV-2 Nucleocapsid Protein
HCoV-229E Nucleocapsid Protein
HCoV-NL63 Nucleocapsid Protein
HCoV-HKU1 Nucleocapsid Protein
HCoV-OC43 Nucleocapsid Protein

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Influenza A nucleoprotein Antibody (010) [Alexa Fluor™ Plus 594]

• Influenza A virus NP
• NP
• NP-A
• Nucleocapsid protein
• Nucleoprotein

Background

The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.