Influenza A H1N1 Hemagglutinin Antibody - BSA Free

Western Blot: Influenza A H1N1 Hemagglutinin Antibody [NBP2-24956] - Analysis of Influenza A H1N1 Hemagglutinin (swine flu) immunizing peptide conjugated to a carrier protein using the NBP2-24956 at 2 ug/ml. A band at ~29 kDa was detected, corresponding to the MW of the immunogen/fusion protein. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

• Reactivity: V
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Influenza A H1N1 Hemagglutinin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Influenza A H1N1 Hemagglutinin Antibody - BSA Free (NBP2-24956) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Within the range of amino acids 238-270 of Influenza A H1N1 NA (swine flu) were used as the immunogen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-3 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Background

nfluenza A H1N1 NA, also known as Neuraminidase, is a protein belonging to the Influenza A virus strain (strain A/Swine/New Jersey/11/1976 H1N1) with 469 amino acids. It belongs to the glycosyl hydrolase 34 family. This Influenza A virus has aves, human and pig as its host. Neuraminidase is a homotetramer located in the virion membrane and host apical cell membrane and preferentially accumulates at the apical plasma membrane in infected polarized epithelial cells, which is the virus assembly site. This protein is a receptor-destroying enzyme that catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates (cellular receptors) during virus budding to facilitate virus release, spreading, prevents self-aggregation of virus and ensures the efficient spread of the progeny virus from cell to cell. It may facilitate viral invasion of the upper airways by cleaving the sialic acid moieties on the mucin of the airway epithelial cells. Neuraminidase plays a role in the determination of host range restriction on replication and virulence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.