![Western Blot: Rabbit anti-Human IgG4 Secondary Antibody (RM120) [Biotin] [NBP2-62126B] - Western Blot of human, mouse, rat, and goat IgG shows NBP2-62126B reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG.](http://images.novusbio.com/fullsize/Rabbit-anti-Human-IgG4-Secondary-Antibody-RM120-Biotin-Western-Blot-NBP2-62126B-img0003.jpg "Western Blot: Rabbit anti-Human IgG4 Secondary Antibody (RM120) [Biotin] [NBP2-62126B] - Western Blot of human, mouse, rat, and goat IgG shows NBP2-62126B reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, ELISA, ICC/IF |
| Clone | RM120 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Biotin |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Peptide corresponding to the hinge region of Human IgG4 |
| Specificity | This antibody reacts to the heavy chain of human IgG4, This antibody does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG3), and shows no cross reactivity to IgM, IgA, IgD, IgE. This antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This primary antibody can also be used as a secondary antibody. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 50% Glycerol/PBS with 1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for IgG4 Antibody (NBP2-62126B)Find related products by research area.
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![Immunohistochemistry: Rabbit anti-Human IgG4 Secondary Antibody (RM120) [Biotin] [NBP2-62126B] - Immunohistochemistry of Human Lymphoid Tissue using Anti-Human IgG4 antibody NBP2-62126B.](http://images.novusbio.com/fullsize/Rabbit-anti-Human-IgG4-Secondary-Antibody-RM120-Biotin-Immunohistochemistry-NBP2-62126B-img0001.jpg "Immunohistochemistry: Rabbit anti-Human IgG4 Secondary Antibody (RM120) [Biotin] [NBP2-62126B] - Immunohistochemistry of Human Lymphoid Tissue using Anti-Human IgG4 antibody NBP2-62126B.")
![ELISA: Rabbit anti-Human IgG4 Secondary Antibody (RM120) [Biotin] [NBP2-62126B] - A titer ELISA using NBP2-62126B. The plate was coated with different amounts of human IgG4. A serial dilution of NBP2-62126B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Human-IgG4-Secondary-Antibody-RM120-Biotin-ELISA-NBP2-62126B-img0002.jpg "ELISA: Rabbit anti-Human IgG4 Secondary Antibody (RM120) [Biotin] [NBP2-62126B] - A titer ELISA using NBP2-62126B. The plate was coated with different amounts of human IgG4. A serial dilution of NBP2-62126B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.")
![ELISA: Rabbit anti-Human IgG4 Secondary Antibody (RM120) [Biotin] [NBP2-62126B] - Sandwich ELISA using NBP2-62126B as the capture antibody, and Biotinylated anti-human light chains (kappa + lambda) antibody as the detection antibody, followed by an AP conjugated streptavidin.](http://images.novusbio.com/fullsize/Rabbit-anti-Human-IgG4-Secondary-Antibody-RM120-Biotin-ELISA-NBP2-62126B-img0004.jpg "ELISA: Rabbit anti-Human IgG4 Secondary Antibody (RM120) [Biotin] [NBP2-62126B] - Sandwich ELISA using NBP2-62126B as the capture antibody, and Biotinylated anti-human light chains (kappa + lambda) antibody as the detection antibody, followed by an AP conjugated streptavidin.")
![ELISA: Rabbit anti-Human IgG4 Secondary Antibody (RM120) [Biotin] [NBP2-62126B] - Sandwich ELISA using NBP2-62126B as the capture antibody, and Biotinylated anti-human light chains (kappa + lambda) antibody as the detection antibody, followed by an AP conjugated streptavidin.](http://images.novusbio.com/fullsize/Rabbit-anti-Human-IgG4-Secondary-Antibody-RM120-Biotin-ELISA-NBP2-62126B-img0005.jpg "ELISA: Rabbit anti-Human IgG4 Secondary Antibody (RM120) [Biotin] [NBP2-62126B] - Sandwich ELISA using NBP2-62126B as the capture antibody, and Biotinylated anti-human light chains (kappa + lambda) antibody as the detection antibody, followed by an AP conjugated streptavidin.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")