![Western Blot: IgG4 Antibody (RM120) - Azide and BSA Free [NBP3-25977] - Western Blot of human, mouse, rat, and goat IgG shows RM120 reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG.](http://images.novusbio.com/fullsize/nbp3-25977_rabbit-igg4-mab-rm120-azide-and-bsa-free-2462024132617.png "Western Blot: IgG4 Antibody (RM120) - Azide and BSA Free [NBP3-25977] - Western Blot of human, mouse, rat, and goat IgG shows RM120 reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, ELISA |
| Clone | RM120 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-62126B | |||
| Unconjugated | NBP2-62126 | |||
| Description | Novus Biologicals Rabbit IgG4 Antibody (RM120) - Azide and BSA Free (NBP2-62126) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Peptide corresponding to the hinge region of Human IgG4 |
| Specificity | This antibody reacts to the heavy chain of human IgG4, This antibody does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG3), and shows no cross reactivity to IgM, IgA, IgD, IgE. This antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: IgG4 Antibody (RM120) - Azide and BSA Free [NBP3-25977] - Immunohistochemistry of Human Lymphoid Tissue using Clone RM120.](http://images.novusbio.com/fullsize/nbp3-25977_rabbit-igg4-mab-rm120-azide-and-bsa-free-2462024132643.jpg "Immunohistochemistry-Paraffin: IgG4 Antibody (RM120) - Azide and BSA Free [NBP3-25977] - Immunohistochemistry of Human Lymphoid Tissue using Clone RM120.")
![ELISA: IgG4 Antibody (RM120) - Azide and BSA Free [NBP3-25977] - A titer ELISA using RM120. The plate was coated with different amounts of human IgG4. A serial dilution of RM120 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp3-25977_rabbit-igg4-mab-rm120-azide-and-bsa-free-elisa-189202523444723.png "ELISA: IgG4 Antibody (RM120) - Azide and BSA Free [NBP3-25977] - A titer ELISA using RM120. The plate was coated with different amounts of human IgG4. A serial dilution of RM120 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")