MAB3286, Mouse IgG_2Bisotype), anti-HSP60 (MAB1800, Mouse IgG_2Bisotype), anti-GAPDH (MAB5718,Mouse IgG₁isotype), anti-Peroxiredoxin 6 (MAB3490, Mouse IgG₃isotype), and anti-SOD1 (MAB3418, Mouse IgG_2Aisotype). The left panel was then probed with 0.5 µg/mL of Rabbit Anti-Mouse IgG_2B(Catalog # MAB9636) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific bands were detected for IgG_2Bisotype antibodies at approximately 60 and 90 kDa (as indicated). The right panel was probed with HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018) but not Rabbit Anti-Mouse IgG_2BMonoclonal Antibody (Catalog # MAB9636). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> MAB3286, Mouse IgG_2Bisotype), anti-HSP60 (MAB1800, Mouse IgG_2Bisotype), anti-GAPDH (MAB5718,Mouse IgG₁isotype), anti-Peroxiredoxin 6 (MAB3490, Mouse IgG₃isotype), and anti-SOD1 (MAB3418, Mouse IgG_2Aisotype). The left panel was then probed with 0.5 µg/mL of Rabbit Anti-Mouse IgG_2B(Catalog # MAB9636) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific bands were detected for IgG_2Bisotype antibodies at approximately 60 and 90 kDa (as indicated). The right panel was probed with HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018) but not Rabbit Anti-Mouse IgG_2BMonoclonal Antibody (Catalog # MAB9636). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 and Jurkat cell lines. PVDF membrane was probed with anti-HSP90 (MAB3286, Mouse IgG_2Bisotype), anti-HSP60 (MAB1800, Mouse IgG_2Bisotype), anti-GAPDH (MAB5718,Mouse IgG₁isotype), anti-Peroxiredoxin 6 (MAB3490, Mouse IgG₃isotype), and anti-SOD1 (MAB3418, Mouse IgG_2Aisotype). The left panel was then probed with 0.5 µg/mL of Rabbit Anti-Mouse IgG_2B(Catalog # MAB9636) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific bands were detected for IgG_2Bisotype antibodies at approximately 60 and 90 kDa (as indicated). The right panel was probed with HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018) but not Rabbit Anti-Mouse IgG_2BMonoclonal Antibody (Catalog # MAB9636). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB100, Mouse IgG₁isotype), Mouse anti-Human CD4 Monoclonal Antibody (MAB3791, Mouse IgG_2Aisotype), or Mouse anti-Human CD8a Monoclonal Antibody (MAB1509, Mouse IgG_2Bisotype), followed by Rabbit anti-Mouse IgG_2B-specific secondary antibody (Catalog # MAB9636) then PE-conjugated Goat anti-Rabbit secondary antibody (F0110). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB100, Mouse IgG₁isotype), Mouse anti-Human CD4 Monoclonal Antibody (MAB3791, Mouse IgG_2Aisotype), or Mouse anti-Human CD8a Monoclonal Antibody (MAB1509, Mouse IgG_2Bisotype), followed by Rabbit anti-Mouse IgG_2B-specific secondary antibody (Catalog # MAB9636) then PE-conjugated Goat anti-Rabbit secondary antibody (F0110). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

MAB859, Mouse IgG₁ isotype; aqua histogram), Mouse anti-Human MICA Monoclonal Antibody (Catalog # MAB13001, Mouse IgG_2A isotype; dark blue histogram), or Mouse anti-Human MICA Monoclonal Antibody (Catalog # MAB1300, Mouse IgG_2B isotype; orange histogram), followed by Rabbit anti-Mouse IgG_2B-specific secondary antibody (Catalog # MAB9636) then APC-conjugated Goat anti-Rabbit secondary antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB859, Mouse IgG₁ isotype; aqua histogram), Mouse anti-Human MICA Monoclonal Antibody (Catalog # MAB13001, Mouse IgG_2A isotype; dark blue histogram), or Mouse anti-Human MICA Monoclonal Antibody (Catalog # MAB1300, Mouse IgG_2B isotype; orange histogram), followed by Rabbit anti-Mouse IgG_2B-specific secondary antibody (Catalog # MAB9636) then APC-conjugated Goat anti-Rabbit secondary antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />