![ELISA: Ig Light Chain Antibody (RM129) - Azide and BSA Free [NBP3-25967] - A titer ELISA using RM129. The plate was coated with different amounts of human IgG3 gamma. A serial dilution of RM129 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp3-25967_rabbit-ig-light-chain-mab-rm129-azide-and-bsa-free-226202422174411.png "ELISA: Ig Light Chain Antibody (RM129) - Azide and BSA Free [NBP3-25967] - A titer ELISA using RM129. The plate was coated with different amounts of human IgG3 gamma. A serial dilution of RM129 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF, IHC |
| Clone | RM129 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Human IgG |
| Specificity | This antibody reacts to both kappa and lambda light chains of human immunoglobulins. The antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Sandwich ELISA: Ig Light Chain Antibody (RM129) - Azide and BSA Free [NBP3-25967] - A titer Sandwich ELISA using Clone RM129 as the detection antibody. The plate, coated with the capture antibody anti-human IgG RM116, was loaded with different amounts of human IgG. A serial dilution of Biotin RM129 was used as the detection antibody, followed with an alkaline phosphatase conjugated streptavidin.](http://images.novusbio.com/fullsize/nbp3-25967_rabbit-ig-light-chain-mab-rm129-azide-and-bsa-free-22620242217445.png "Sandwich ELISA: Ig Light Chain Antibody (RM129) - Azide and BSA Free [NBP3-25967] - A titer Sandwich ELISA using Clone RM129 as the detection antibody. The plate, coated with the capture antibody anti-human IgG RM116, was loaded with different amounts of human IgG. A serial dilution of Biotin RM129 was used as the detection antibody, followed with an alkaline phosphatase conjugated streptavidin.")
![ELISA: Ig Light Chain Antibody (RM129) - Azide and BSA Free [NBP3-25967] - ELISA shows that clone RM129 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM129 and a monkey IgG reacting antibody (positive control) was used as the detection antibodies.](http://images.novusbio.com/fullsize/nbp3-25967_rabbit-ig-light-chain-mab-rm129-azide-and-bsa-free-226202422321246.png "ELISA: Ig Light Chain Antibody (RM129) - Azide and BSA Free [NBP3-25967] - ELISA shows that clone RM129 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM129 and a monkey IgG reacting antibody (positive control) was used as the detection antibodies.")
![ELISA: Ig Light Chain Antibody (RM129) - Azide and BSA Free [NBP3-25967] - A titer ELISA using RM129. The plate was coated with different amounts of human IgG4k. A serial dilution of RM129 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp3-25967_rabbit-ig-light-chain-mab-rm129-azide-and-bsa-free-24620241245918.png "ELISA: Ig Light Chain Antibody (RM129) - Azide and BSA Free [NBP3-25967] - A titer ELISA using RM129. The plate was coated with different amounts of human IgG4k. A serial dilution of RM129 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.")
![ELISA: Ig Light Chain Antibody (RM129) - Azide and BSA Free [NBP3-25967] - ELISA of human immunoglobulins shows RM129 reacts to both kappa and lambda light chains of human immunoglobulins. No cross reactivity with mouse, rat, or goat immunoglobulin light chain. The plate was coated with 50 ng/well of different immunoglobulins. 100 ng/mL or 20 ng/mL of RM129 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp3-25967_rabbit-ig-light-chain-mab-rm129-azide-and-bsa-free-22620242311072.png "ELISA: Ig Light Chain Antibody (RM129) - Azide and BSA Free [NBP3-25967] - ELISA of human immunoglobulins shows RM129 reacts to both kappa and lambda light chains of human immunoglobulins. No cross reactivity with mouse, rat, or goat immunoglobulin light chain. The plate was coated with 50 ng/well of different immunoglobulins. 100 ng/mL or 20 ng/mL of RM129 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")