![Sandwich ELISA: Ig Light Chain Antibody (RM129) [Biotin] [NBP3-18532] - Detection of human IgG1 in monkey serum, NBP2-62028 (capture) and NBP3-18532 (detection) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.](http://images.novusbio.com/fullsize/Ig-Light-Chain-Antibody-RM129-Biotin-Sandwich-ELISA-NBP3-18532-img0001.jpg "Sandwich ELISA: Ig Light Chain Antibody (RM129) [Biotin] [NBP3-18532] - Detection of human IgG1 in monkey serum, NBP2-62028 (capture) and NBP3-18532 (detection) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF, IHC |
| Clone | RM129 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Biotin |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal |
| Immunogen | Human IgG |
| Specificity | This antibody reacts to both kappa and lambda light chains of human immunoglobulins. The antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at -20C. |
| Buffer | 50% Glycerol, PBS, 1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Sandwich ELISA: Ig Light Chain Antibody (RM129) [Biotin] [NBP3-18532] - A titer Sandwich ELISA using NBP3-18532 as the detection antibody. The plate, coated with the capture antibody Clone RM116, was loaded with different amounts of human IgG. A serial dilution of NBP3-18532 was used as the detection antibody, followed with an alkaline phosphatase conjugated streptavidin.](http://images.novusbio.com/fullsize/nbp3-18532_rabbit-igg-light-chain-mab-rm129-biotin-22620242217444.png "Sandwich ELISA: Ig Light Chain Antibody (RM129) [Biotin] [NBP3-18532] - A titer Sandwich ELISA using NBP3-18532 as the detection antibody. The plate, coated with the capture antibody Clone RM116, was loaded with different amounts of human IgG. A serial dilution of NBP3-18532 was used as the detection antibody, followed with an alkaline phosphatase conjugated streptavidin.")
![ELISA: Ig Light Chain Antibody (RM129) [Biotin] [NBP3-18532] - ELISA showing NBP3-18532 reacts only to kappa and lambda light chain of all human immunoglobulins, not to mouse IgG, rat IgG, or goat IgG.](http://images.novusbio.com/fullsize/Ig-Light-Chain-Antibody-RM129-Biotin-ELISA-NBP3-18532-img0002.jpg "ELISA: Ig Light Chain Antibody (RM129) [Biotin] [NBP3-18532] - ELISA showing NBP3-18532 reacts only to kappa and lambda light chain of all human immunoglobulins, not to mouse IgG, rat IgG, or goat IgG.")
![ELISA: Ig Light Chain Antibody (RM129) [Biotin] [NBP3-18532] - ELISA showing NBP3-18532 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of NBP3-18532 and a monkey IgG binding antibody (positive control) was used as the detection antibody.](http://images.novusbio.com/fullsize/Ig-Light-Chain-Antibody-RM129-Biotin-ELISA-NBP3-18532-img0003.jpg "ELISA: Ig Light Chain Antibody (RM129) [Biotin] [NBP3-18532] - ELISA showing NBP3-18532 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of NBP3-18532 and a monkey IgG binding antibody (positive control) was used as the detection antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")