Recombinant Human IDH3G His Protein

SDS-Page: Recombinant Human IDH3G His Protein [NBP1-78823] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human IDH3G His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 40-393 of Human IDH3G
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MFSEQTIPPS AKYGGRHTVT MIPGDGIGPE LMLHVKSVFR HACVPVDFEE VHVSSNADEE DIRNAIMAIR RNRVALKGNI ETNHNLPPSH KSRNNILRTS LDLYANVIHC KSLPGVVTRH KDIDILIVRE NTEGEYSSLE HESVAGVVES LKIITKAKSL RIAEYAFKLA QESGRKKVTA VHKANIMKLG DGLFLQCCRE VAARYPQITF ENMIVDNTTM QLVSRPQQFD VMVMPNLYGN IVNNVCAGLV GGPGLVAGAN YGHVYAVFET ATRNTGKSIA NKNIANPTAT LLASCMMLDH LKLHSYATSI RKAVLASMDN ENMHTPDIGG QGTTSEAIQD VIRHIRVING RAVEA

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IDH3G
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 41.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 50% glycerol, 0.2 M NaCl, 5mM DTT, 2 mM EDTA
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human IDH3G His Protein

• EC 1.1.1
• EC 1.1.1.41
• H-IDHG
• IDH-gamma
• isocitrate dehydrogenase 3 (NAD+) gamma
• isocitrate dehydrogenase, NAD(+)-specific, mitochondrial, gamma subunit
• mitochondrial
• NAD (H)-specific isocitrate dehydrogenase gamma subunit
• NAD+-specific ICDH

Background

IDH3G, also known as isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, belongs to the isocitrate and isopropylmalate dehydrogenases family. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. IDH3G is a gamma subunit of one isozyme of isocitrate dehydrogenase that belongs to a distinct subclass, which utilizes NAD(+) as the electron acceptor, and is localised to the mitochondrial matrix. Recombinant human IDH3G protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IDH3G