IDH3G Overexpression Lysate (Native)


Western Blot: IDH3G Overexpression Lysate (Adult Normal) [NBL1-11821] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IDH3G.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

IDH3G Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC201692 and based on accession number NM_004135. The protein contains a C-terminal DDK tag.
Homo sapiens isocitrate dehydrogenase 3 (NAD+) gamma (IDH3G), nuclear gene encoding mitochondrial protein, transcript variant 1, mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
38.8 kDa

Lysate Details for IDH3G

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for IDH3G Overexpression Lysate (Native)

  • EC 1.1.1
  • EC
  • H-IDHG
  • IDH-gamma
  • isocitrate dehydrogenase 3 (NAD+) gamma
  • isocitrate dehydrogenase, NAD(+)-specific, mitochondrial, gamma subunit
  • mitochondrial
  • NAD (H)-specific isocitrate dehydrogenase gamma subunit
  • NAD+-specific ICDH


Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants of this gene have been described, but only some of their full length natures have been determined. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longer isoform (a).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Mu
Applications: WB, IHC
Applications: WB

Publications for IDH3G Lysate (NBL1-11821) (0)

There are no publications for IDH3G Lysate (NBL1-11821).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IDH3G Lysate (NBL1-11821) (0)

There are no reviews for IDH3G Lysate (NBL1-11821). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IDH3G Lysate (NBL1-11821) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IDH3G Overexpression Lysate (Native) Products

Related Products by Gene

Bioinformatics Tool for IDH3G Lysate (NBL1-11821)

Discover related pathways, diseases and genes to IDH3G Lysate (NBL1-11821). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on IDH3G

There are no specific blogs for IDH3G, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol IDH3G

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBL1-11821 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

IDH3G Antibody
IDH3G Antibody
IDH3G Antibody