HSP70/DnaK Antibody (8E2/2)

Western Blot: HSP70/DnaK Antibody (8E2/2) [NBP1-97490] - HSP70/HSPA1A Antibody (8E2/2) [NBP1-97490] - Analysis: Lane 1: MW Marker, Lane 2: DnaK active recombinant protein, Lane 3: E. coli cell lysate.

• Reactivity: Hu, Ec
• Applications: WB
• Clone: 8E2/2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

HSP70/DnaK Antibody (8E2/2) Summary

• Immunogen: E. coli DnaK.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: dnaK
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:10000
• Application Notes: Use in Western Blot reported in scientific literature (PMID:33918708)
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Human reported in scientific literature (PMID:33918708)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for HSP70/DnaK Antibody (8E2/2)

• chaperone protein DnaK
• ECK0014
• groPAB
• groPC
• groPF
• grpC
• grpF
• SEG

Background

DnaK possesses an autophosphorylation activity and a weak 5-nucleotidase activity, cleaving the 5 phosphate groups from both ribose and deoxyribose nucleotides. The role of DnaK in ATP-dependent protein-protein interactions has been extended to normal E. coli physiology, where, like eukaryotic Hsp70 homologs, it is thought to participate in the assembly/disassembly of protein complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSP70/DnaK Antibody (NBP1-97490)(1)

Publication using NBP1-97490

• Curreli S, Tettelin H, Benedetti F et al. Analysis of DnaK Expression from a Strain of Mycoplasma fermentans in Infected HCT116 Human Colon Carcinoma Cells International Journal of Molecular Sciences 2021-04-09 [PMID: 33918708] (WB, Human)

Bioinformatics

• Gene Symbol: dnaK
• Uniprot:
  • P08107()
  • P0A6Y8(E. coli)