Histone H2B Antibody

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Product Details

Summary
Reactivity BvSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated

Order Details

Histone H2B Antibody Summary

Immunogen
Intact calf H-2B complexed to rRNA.
Localization
Nuclear
Specificity
This is specific for Histone 2B.
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
HIST2H2BF
Purity
Protein G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 1:1000
  • Western Blot 1:1000

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for Histone H2B Antibody

  • FLJ35099
  • FLJ56780
  • FLJ56787
  • HIST1H2BK
  • histone 2, H2bf
  • histone cluster 2, H2bf
  • histone H2B type 2-F
  • Histone H2B
  • MGC131639

Background

In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3 and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in the formation of higher order structure in chromatin and the maintenance of overall chromatin compaction. Whilst the core histones are highly conserved across a wide range of organisms, the linker histones are less conserved.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HIST2H2BF