Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) - BSA Free

Western Blot: Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) [NBP2-59149] - Histone H2B Antibody (C.15200232) [NBP2-59149] - Analysis on whole cell extracts from WT yeast cells (lane 1), yeast cells with higher steady state levels of H2BK123ub (lane 2), none ubiquitinylated H2B (lane 3) and ubiquitinylated H2A (lane 4). The position of the protein of interest is indicated on the right.

Chromatin Immunoprecipitation: Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) [NBP2-59149] - Histone H2B Antibody (C.15200232) [NBP2-59149] - ChIP assays were performed using human HeLa cells, the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the coding regions of the active CCT5 and EIF2S3 genes, used as positive controls, and for the inactive TSH2B gene and the Sat2 satellite repeat region used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Western Blot: Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) [NBP2-59149] - Histone H2B Antibody (C.15200232) [NBP2-59149] - Analysis on histone extracts from HeLa cells (15 ug) using the antibody against H2BK123ub. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.

Chromatin Immunoprecipitation: Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) [NBP2-59149] - ChIP results obtained with the antibody against H2BK123ub on sheared chromatin from WT yeast cells, yeast cells with higher steady state levels of H2BK123ub and yeast cells with no ubiquitinylated H2B. Quantitative PCR was performed with primers for the coding region of an active gene. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

• Reactivity: Hu, Ye
• Applications: WB, ChIP
• Clone: C.15200232
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) - BSA Free Summary

• Description: Novus Biologicals Mouse Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) - BSA Free (NBP2-59149) is a monoclonal antibody validated for use in WB and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The exact immunogen is propietary information.
• Modification: ubiquitylated Lys123
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: H2BC18
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 2 ug/IP
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) - BSA Free

• FLJ35099
• FLJ56780
• FLJ56787
• HIST1H2BK
• histone 2, H2bf
• histone cluster 2, H2bf
• histone H2B type 2-F
• Histone H2B
• MGC131639

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility tothe cellular machineries which require DNA as a template. Histones thereby play a central role in transcriptionregulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set ofpost-translational modifications of histones, also called histone code, and nucleosome remodeling

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H2BC18