HIST1H2AG Antibody (CL5039) - Azide and BSA Free

Staining of human kidney shows strong nuclear positivity in cells in glomeruli and tubules.

Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Analysis in human cell line K562.

Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Analysis of purified human recombinant histone H2A, H2A.X, H2A.Z, mH2A.1, mH2A.2, H2B, H3 and H4.

Staining of human small intestine shows strong nuclear positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL5039
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

HIST1H2AG Antibody (CL5039) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse HIST1H2AG Antibody (CL5039) - Azide and BSA Free (NBP2-61151) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of P0C0S8, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HIST1H2AG
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for HIST1H2AG Antibody (CL5039) - Azide and BSA Free

• H2A histone family, member P
• H2A.1
• H2A.1b
• H2A/p
• H2AFC
• H2AFD
• H2AFI
• H2AFN
• H2AFP
• HIST1H2AI
• HIST1H2AK
• HIST1H2AL
• HIST1H2AM
• histone 1, H2ag
• histone cluster 1, H2ag
• histone H2A type 1
• Histone H2A/p
• pH2A/f

Background

HIST1H2AG is 130 amino acids long, weighs approximately 14 kDa, and the gene codes for a part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Current studies are being done on several diseases and disorders including riddle syndrome, systemic lupus erythematosus, lupus erythematosus, schizophrenia, and immunodeficiency. HIST1H2AG has also been shown to have interactions with IL33, RYBP, YWHAZ, YWHAQ, and SAP30 in pathways such as the histone modification, signal transduction of Activin A, and systemic lupus erythematosus pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HIST1H2AG