HIST1H2AB [ubiquitylated Lys119] Antibody - Azide and BSA Free

Western Blot: HIST1H2AB [ubiquitylated Lys119] Antibody - Azide and BSA Free [HIST1H2AB] - Western blot analysis of lysates from C6 cells, using HIST1H2AB Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 180s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HIST1H2AB [ubiquitylated Lys119] Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit HIST1H2AB [ubiquitylated Lys119] Antibody - Azide and BSA Free (NBP3-15563) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic ubiquitinated peptide around K119 of human HIST1H2AB (NP_734466.1). LPKKT
• Modification: ubiquitylated Lys119
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: H2AC4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for HIST1H2AB [ubiquitylated Lys119] Antibody - Azide and BSA Free

• histone H2A type 1-B/E
• H2A/m
• H2AFM
• histone cluster 1, H2ab

Background

HIST1H2AB is 130 amino acids long, weighs approximately 14 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Current studies are being done on several diseases and disorders including riddle syndrome, systemic lupus erythematosus, lupus erythematosus, cockayne syndrome, hemochromatosis, and immunodeficiency. HIST1H2AB has also been shown to have interactions with HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4E in pathways such as the proteasome degradation, telomere maintenance, nucleosome assembly, cell cycle, and systemic lupus erythematosus pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: H2AC4