![Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody - Azide and BSA Free [NBP2-33100] - Formalin-fixed. paraffin-embedded human stomach stained with helicobacter pylori rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Helicobacter-Pylori-Antibody---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33100-img0001.jpg "Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody - Azide and BSA Free [NBP2-33100] - Formalin-fixed. paraffin-embedded human stomach stained with helicobacter pylori rabbit polyclonal antibody.")
| Reactivity | BaSpecies Glossary |
| Applications | IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-29479). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Total sonicate of Helicobacter pylori |
| Localization | Cell surface and cytoplasm |
| Specificity | The spiral shaped bacterium Helicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy.H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody - Azide and BSA Free [NBP2-33100] - Formalin-fixed, paraffin-embedded human stomach stained with Helicobacter pylori antibody (1:200), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues requires boiling tissue sections in citrate buffer, pH 6, or 1 mM EDTA, pH 8.0](http://images.novusbio.com/fullsize/Helicobacter-Pylori-Antibody-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33100-img0002.jpg "Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody - Azide and BSA Free [NBP2-33100] - Formalin-fixed, paraffin-embedded human stomach stained with Helicobacter pylori antibody (1:200), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues requires boiling tissue sections in citrate buffer, pH 6, or 1 mM EDTA, pH 8.0")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")