![ELISA: H1N1 Neuraminidase Antibody [NBP2-41110] - ELISA results using H1N1 Neuraminidase antibody at 1 ug/mL and the blocking and corresponding peptides at 50, 10, 2 and 0 ng/mL.](http://images.novusbio.com/fullsize/H1N1-Neuraminidase-Antibody-ELISA-NBP2-41110-img0001.jpg "ELISA: H1N1 Neuraminidase Antibody [NBP2-41110] - ELISA results using H1N1 Neuraminidase antibody at 1 ug/mL and the blocking and corresponding peptides at 50, 10, 2 and 0 ng/mL.")
| Reactivity | V, V-ViSpecies Glossary |
| Applications | ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against synthetic peptides from the seasonal influenza (H1N1) Neuraminidase protein and the novel swine influenza Neuraminidase protein. The antibody recognizes both variants. The immunogen is located within the first 50 amino acids of H1N1 Neuraminidase. Amino Acid Squence: SIQLGNQNQIET |
| Specificity | This antibody will recognize the Neuraminidase from the both the seasonal influenza (H1N1) and swine-origin influenza virus (A/California/14/2009 (H1N1)). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: H1N1 Neuraminidase Antibody - BSA Free [NBP2-41110] - Validation with H1N1 NA Protein. Loading: 50ng (Lane 1) and 100ng (Lane 2) H1N1 Neuraminidase recombinant protein. Antibodies: H1N1 NA antibody, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.](http://images.novusbio.com/fullsize/nbp2-41110_rabbit-polyclonal-h1n1-neuraminidase-antibody-2711202419201590.gif "Western Blot: H1N1 Neuraminidase Antibody - BSA Free [NBP2-41110] - Validation with H1N1 NA Protein. Loading: 50ng (Lane 1) and 100ng (Lane 2) H1N1 Neuraminidase recombinant protein. Antibodies: H1N1 NA antibody, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.")
![ELISA: H1N1 Neuraminidase Antibody - BSA Free [NBP2-41110] - Figure 2 ELISA Validation with H1N1 NA Protein Coating Antigen: H1N1 Neuraminidase recombinant protein, 2 u/mL, incubated at 4 C overnight. Detection Antibodies: H1N1 NA antibody, , dilution: 1-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit at 1:10,000, incubated at RT for 1 hr.](http://images.novusbio.com/fullsize/nbp2-41110_rabbit-polyclonal-h1n1-neuraminidase-antibody-2711202420171756.jpg "ELISA: H1N1 Neuraminidase Antibody - BSA Free [NBP2-41110] - Figure 2 ELISA Validation with H1N1 NA Protein Coating Antigen: H1N1 Neuraminidase recombinant protein, 2 u/mL, incubated at 4 C overnight. Detection Antibodies: H1N1 NA antibody, , dilution: 1-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit at 1:10,000, incubated at RT for 1 hr.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")