GDAP1L1 Antibody (OTI1G5) - Azide and BSA Free

Western Blot: GDAP1L1 Antibody (OTI1G5) - Azide and BSA Free [NBP2-72182] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GDAP1L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GDAP1L1.

Immunocytochemistry/Immunofluorescence: GDAP1L1 Antibody (OTI1G5) - Azide and BSA Free [NBP2-72182] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GDAP1L1.

Flow Cytometry: GDAP1L1 Antibody (OTI1G5) - Azide and BSA Free [NBP2-72182] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-GDAP1L1 antibody, and then analyzed by flow cytometry.

Western Blot: GDAP1L1 Antibody (OTI1G5) - Azide and BSA Free [NBP2-72182] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GDAP1L1 monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, CyTOF-ready
• Clone: OTI1G5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

GDAP1L1 Antibody (OTI1G5) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human GDAP1L1(NP_076939) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GDAP1L1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000-2000
• Theoretical MW: 41.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for GDAP1L1 Antibody (OTI1G5) - Azide and BSA Free

• dJ881L22.1
• dJ995J12.1.1
• DKFZp761K228
• ganglioside-induced differentiation-associated protein 1-like 1
• GDAP1-L1

Background

The ganglioside GD3 synthase causes cell differentiation with neurite sprouting when transfected into the mouse neuroblastoma cell line Neuro2a. After differentiation, the expression of several genes is upregulated, including one that encodes a protein termed ganglioside-induced differentiation-associated protein 1 (Gdap1). A similar gene was found in humans, and mutations in the human gene are associated with Charcot-Marie-Tooth type 4A disease. The protein encoded by this gene is similar in sequence to the human GDAP1 protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GDAP1L1