Fanconi Anemia Antibody Pack

Western Blot: Fanconi Anemia Antibody Pack 4vials [NBP1-78946] - Detection of tankyrase in DU145 whole cell lysates (50 ug) using NB 100-186. ECL detection - 30 seconds. Lane 1 - 1:500 primary dilution Lane 2 - 1:2,000 primary dilution

Immunocytochemistry/Immunofluorescence: Fanconi Anemia Antibody Pack 4vials [NBP1-78946] - HeLa cells stained NB110-57130 TRF2 Antibody (Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).

Immunohistochemistry-Paraffin: Fanconi Anemia Antibody Pack 4vials [NBP1-78946] - IHC staining of FANCD2 in human prostate, glandular epithelium using NB100-182.

Western Blot: Fanconi Anemia Antibody Pack 4vials [NBP1-78946] - Western blot detection of TRF1 in HeLa whole cell extracts using NB110-68281.

Product Discontinued

Fanconi Anemia Antibody Pack Summary

• Description: 25 ul vials of each of the following: NB100-182, NB110-68281, NB110-57130, NB100-186
• Immunogen: See individual datasheets.
• Specificity: This Fanconi Anemia Antibodies Sample Pack includes antibodies to FANCD2, TRF1, TRF2 and Tankyrase.

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Mainly Western blot and Immunocytochemistry, however additional applications may be possible for individual products.

Reactivity Notes

Human and mouse, however additional species may be possible for individual products.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: See individual datasheets.

Background

Fanconi anemia (FA) is a genetic disease that results from a defect in a group of proteins responsible for DNA repair. As the defect occurs in proteins responsible for DNA repair, many patients develop cancer as a symptom. FA results in decreased production of all types of blood cells. The disease is named after Swiss pediatrician Guido Fanconi who originally described the disorder.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.