![Western Blot: FAM122B Antibody [NBP1-90508] - Analysis in control (vector only transfected HEK293T lysate) and FAM122B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/FAM122B-Antibody-Western-Blot-NBP1-90508-img0009.jpg "Western Blot: FAM122B Antibody [NBP1-90508] - Analysis in control (vector only transfected HEK293T lysate) and FAM122B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: EPDTSYGGTLRRSSSAPLIHGLSDLSQVFQPYTLRTRRNSTTIMSRHSLEEGLDMVNRETAHEREMQTAMQISQSWDESLSLSDSDFDKPEKLYSPKRIDFTPVSPAPSPTRGFGKMFVSSS |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | FAM122B |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NBP1-90508 | Applications | Species |
|---|---|---|
| Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | FAM122B |
![Immunocytochemistry/Immunofluorescence: FAM122B Antibody [NBP1-90508] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FAM122B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90508-img0005.jpg "Immunocytochemistry/Immunofluorescence: FAM122B Antibody [NBP1-90508] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: FAM122B Antibody [NBP1-90508] - Staining in human endometrium and liver tissues using anti-FAM122B antibody. Corresponding FAM122B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FAM122B-Antibody-Immunohistochemistry-Paraffin-NBP1-90508-img0008.jpg "Immunohistochemistry-Paraffin: FAM122B Antibody [NBP1-90508] - Staining in human endometrium and liver tissues using anti-FAM122B antibody. Corresponding FAM122B RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: FAM122B Antibody [NBP1-90508] - Staining of human endometrium shows high expression.](http://images.novusbio.com/fullsize/FAM122B-Antibody-Immunohistochemistry-Paraffin-NBP1-90508-img0006.jpg "Immunohistochemistry-Paraffin: FAM122B Antibody [NBP1-90508] - Staining of human endometrium shows high expression.")
![Immunohistochemistry-Paraffin: FAM122B Antibody [NBP1-90508] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/FAM122B-Antibody-Immunohistochemistry-Paraffin-NBP1-90508-img0007.jpg "Immunohistochemistry-Paraffin: FAM122B Antibody [NBP1-90508] - Staining of human liver shows low expression as expected.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")