Essential iPSC Factor Antibody Pack

Images

 
Western Blot: Essential iPSC Factor Antibody Pack [NBP1-42824] - analysis using anti-LIN28 mAb against NTERA-2 cell lysate (1). NBP1-28865
Immunohistochemistry-Paraffin: Essential iPSC Factor Antibody Pack [NBP1-42824] - Human Colon: Formalin-Fixed Paraffin Embedded (FFPE) in NBP1-39760
Flow Cytometry: Essential iPSC Factor Antibody Pack [NBP1-42824] - FACS staining of NTERA-2 cells using NB100-588 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody.
Western Blot: Essential iPSC Factor Antibody Pack [NBP1-42824] - Detection of SOX2 in mouse brain lysate using NB110-37235 (0.5ug/ml).
Immunoprecipitation: Essential iPSC Factor Antibody Pack [NBP1-42824] - WB and IP: Nanog Antibody NBP1-42824-Detection of Mouse Nanog on HeLa whole cell lysate using NB100-588. Nanog was also IPed using NB100-587 and ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, Bv, Ch, Pm, ShSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Host
Rabbit

Order Details

Essential iPSC Factor Antibody Pack Summary

Description
Antibody Immunogens NBP2-24749: A portion of amino acids 1-50 of human KLF4 was used as the immunogen for this antibody

NB100-2379: A synthetic peptide made to an internal portion of the human OCT4 protein (between residues 100-200)

NB100-588: A synthetic peptide which maps to a region between residues 250 and the C-terminus (residue 305) of mouse Nanog homeobox

NB600-302: A synthetic peptide corresponding to amino acids 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) of human c-Myc

NB110-37235: A synthetic peptide made to the N-terminal region of human SOX2 protein (within residues 1-100)

NBP1-28865: Purified recombinant fragment of LIN28 (aa93-209) expressed in E. Coli

Kit Type
Kit
Host
Rabbit
Purity
Immunogen affinity purified

Reactivity Notes

NB100-588-Mouse. Reactivity with human Nanog is unlikely owing to divergence of sequence between this ortholog and mouse Nanog at the site to which the epitope maps. NB100-2379-Reacts with human, monkey and mouse. 93% sequence identity with cow and pig proteins NB110-37235 - Human, sheep and mouse. 92% sequence identity with chicken, 86% sequence identity with Xenopus, and 71% sequence identity with Zebrafish proteins.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Kit Components

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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