DYKDDDDK Epitope Tag Antibody (L5) [Biotin]


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Product Details

Reactivity Hu, Dr, NASpecies Glossary
Applications WB, ICC/IF, IP
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Available Conjugates

DYKDDDDK Epitope Tag Antibody (L5) [Biotin] Summary

N-terminal DYKDDDDK-tagged extracellular domain of mouse Langerin. Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody.
Tissue culture supernatant
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Packaging, Storage & Formulations

Store at 4C in the dark.
Biotin (A=N/A, E=N/A)
0.05% Sodium Azide
Tissue culture supernatant


  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10
  • Immunoprecipitation 1:10-1:500
Application Notes
This DYKDDDDK Epitope Tag antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot and Immunohistochemistry. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24454782)
Readout System

Reactivity Notes

Tag specific. Drosophila reactivity reported in scientific literature (PMID: 26573957).

Alternate Names for DYKDDDDK Epitope Tag Antibody (L5) [Biotin]

  • DDDDK epitope tag
  • DDK
  • DYKDDDDK epitope tag
  • DYKDDDDK tag
  • ECS epitope tag
  • ECS tag
  • Enterokinase Cleavage Site epitope tag
  • Enterokinase Cleavage Site tag
  • epitope tag flag
  • FLAG (FLAG is a trademark of Sigma Aldrich)
  • FLAG Tag
  • xxxDDDDK epitope tag


This antibody is a rat monoclonal DYKDDDDK antibody that is a useful tool for the localization and characterization of DDDDK tagged proteins. Testing shows that this monoclonal antibody has 2-8 times more sensitivity than the standard FLAG antibody(TM) epitope tag antibody (1).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DYKDDDDK Epitope Tag Antibody (NBP1-06712B). (Showing 1 - 6 of 6 FAQ).

  1. Are the FLAG Tag antibodies validated in Simple Western?
    • Yes, we offer a 2 FLAG Tag antibodies that have been tested in Simple Western: NB600-345 and NBP1-71705.
  2. Does FLAG Tag antibodies comes in lyophilized form?
    • The following FLAG Tag antibodies come in lyophilized form: NBP1-97401, NBP1-97399, NBP1-97400, NBP1-97402.
  3. What the theoretical molecular weight for FLAG Tag antibodies?
    • The TMW of FLAG Tag antibodies is approximately 150 kDa.
  4. Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.
    • If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.
  5. Will FLAG Tag antibodies recognizes FLAG at the C-terminal end?
    • Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.
  6. What is the immunogen sequence of this FLAG Tag antibody?
    • A sequence within extracellular domain of Mouse Langerin. Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody. The exact sequence is proprietary.

Secondary Antibodies


Isotype Controls

Other Available Formats

DyLight 488 Labeled NBP1-06712G
DyLight 550 Labeled NBP1-06712R
DyLight 650 Labeled NBP1-06712C

Additional DYKDDDDK Epitope Tag Products

DYKDDDDK Epitope Tag NBP1-06712B

Research Areas for DYKDDDDK Epitope Tag Antibody (NBP1-06712B)

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Blogs on DYKDDDDK Epitope Tag.

Hoist the DYKDDDDK Epitope Tag
The DYKDDDDK Epitope Tag antibody was raised against a short, artificial sequence that is widely and commonly used as a protein fusion tag. It is pervasively present in a vast amount of scientific studies, and is a very powerful investigative research...  Read full blog post.

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