DUX4 Antibody (2142A) [Unconjugated]

Images

 
DUX4 was detected in immersion fixed paraffin-embedded sections of human testis using Rabbit Anti-Human DUX4 Monoclonal Antibody (Catalog # MAB9535) at 3 µg/mL for 1 hour at room temperature followed by ...read more
Western blot shows lysates of C2C12 mouse myoblast cell line transfected with human DUX4 untreated (-) or treated (+) with Doxycycline. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human DUX4 ...read more
Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human DUX4 and untreated (-) or treated (+) with Doxycycline, loaded at 0.2 mg/mL. A ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clone
2142A
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

DUX4 Antibody (2142A) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Human DUX4 synthetic peptide
Specificity
Detects human DUX4 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Simple Western 20 ug/mL
  • Immunohistochemistry 3-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DUX4 Antibody (2142A) [Unconjugated]

  • double homeobox 4
  • double homeobox protein 10
  • double homeobox protein 4
  • double homeobox protein 4/10
  • double homeobox protein DUX10
  • DUX10
  • DUX4
  • DUX4L1

Background

Human Double homeobox 4 (aka DUX4) is a protein encoded by the DUX4 gene. This gene is located within a D4Z4 repeat array in chromosome 4q35. Each D4Z4 repeat unit has an open reading frame, DUX4, that contains two homeoboxes. Unregulated expression of DUX4 in muscle cells is the cause of facioscapulohumeral muscular dystrophy (FSHD), a common form of muscular dystrophy in adults that is one of the most prevalent genetic diseases of muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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