dsDNA Antibody (DSD/4054R)

Images

 
Immunohistochemistry-Paraffin: dsDNA Antibody (DSD/4054R) [NBP3-07302] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with dsDNA Recombinant Rabbit Monoclonal Antibody (DSD/4054R).
Immunohistochemistry-Paraffin: dsDNA Antibody (DSD/4054R) [NBP3-07302] - Formalin-fixed, paraffin-embedded human tonsil stained with dsDNA Recombinant Rabbit Monoclonal Antibody (DSD/4054R).
Simple Western: dsDNA Antibody (DSD/4054R) [NBP3-07302] - Lane view of AAV samples probed with NBP3-07302 in Probe 1 of RePlex with chemiluminescence detection and the anti-VP1/2/3 antibody in Probe 2 of RePlex with NIR ...read more
Simple Western: dsDNA Antibody (DSD/4054R) [NBP3-07302] - Lane view of AAV9 samples probed with the anti-DNA antibody in Probe 1 of RePlex with chemiluminescence detection and the anti-VP1/2/3 antibody in Probe 2 of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Simple Western, Flow, ICC/IF, IHC, IHC-P
Clone
DSD/4054R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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Catalog# & Formulation Size Price

dsDNA Antibody (DSD/4054R) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Nuclei of Burkitt's cells
Localization
Nuclear
Marker
Nuclear Marker
Specificity
This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This monoclonal antibody recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This monoclonal antibody produces a homogeneous staining pattern in the nucleus of normal and malignant cells. Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Simple Western
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Publications
Read Publication using NBP3-07302.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA % 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml.(NBP3-08490)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for dsDNA Antibody (DSD/4054R)

  • Double stranded DNA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for dsDNA Antibody (NBP3-07302)(1)

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for dsDNA Antibody (NBP3-07302) (0)

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