DDR1/DDR2 [p Tyr740] Antibody (1119D) [Unconjugated]

Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human DDR2 untreated (-) or treated (+) with 100 nM Calyculin A (Catalog # 1336) for 10 minutes. PVDF membrane was probed with 0.1 μg/mL of Rabbit Anti-Human Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) Monoclonal Antibody (Catalog # MAB25382) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-DDR2 (Y740) at approximately 120-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with human DDR2 untreated (-) or treated (+) with 100 nM Calyculin A (Catalog # 1336) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-DDR2 (Y740) at approximately 130 kDa (as indicated) using 1 μg/mL of Rabbit Anti-Human Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) Monoclonal Antibody (Catalog # MAB25382). This experiment was conducted under reducing conditions and using the 66‑440 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 1119D
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

DDR1/DDR2 [p Tyr740] Antibody (1119D) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Phosphopeptide containing the human DDR2 Y740 site
  Accession # Q16832
• Modification: p Tyr740
• Specificity: Detects human DDR1 and DDR2 when phosphorylated at Y796 and Y740.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DDR2

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Simple Western 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DDR1/DDR2 [p Tyr740] Antibody (1119D) [Unconjugated]

• DDR1/DDR2

Background

DDR2, also known as TYR010 and TKT, is a widely expressed 130 kDa type I transmembrane glycoprotein belonging to the discoidin-like domain containing subfamily of receptor tyrosine kinases (1). Mature human DDR2 consists of a 378 amino acid (aa) extracellular domain (ECD) that includes the discoidin-like domain, a 22 aa transmembrane segment, and a 434 aa cytoplasmic domain that includes the kinase domain (2). Within the ECD, human DDR2 shares 53% aa sequence identity with DDR1 and 97% aa sequence identity with mouse DDR2. The discoidin-like domain mediates DDR2 interactions with collagens I, III, and X (3-5). Collagens II and V are less efficacious ligands (3). DDR2 selectively recognizes the triple helical structure of collagen compared to monomeric or denatured collagen (3, 5, 6). Within collagen II, the D2 period is required for DDR2 binding, and the D1 period is additionally required to trigger DDR2 autophosphorylation (6). The ECD of DDR2 exists as a non-covalent dimer in solution, and dimerization of the receptor greatly enhances collagen binding (4, 7). DDR2 interaction with collagen I inhibits collagen fibrillogenesis and alters collagen fiber morphology (7). Ligand binding induces DDR2 autophosphorylation in the cytoplasmic domain (3, 5, 8), which promotes associations with Shc and Src (9). In addition to the above mechanism, DDR2 exhibits a distinct interaction with collagen X. A region other than the discoidin-like domain of DDR2 recognizes the non-helical NC1 domain of collagen X, and this interaction does not lead to receptor autophosphorylation (5). Activation of DDR2 by collagen induces upregulation of MMP-1, -2, and -13 as well as DDR2 itself (3, 8, 10). DDR2 is implicated in collagenous matrix destruction and cell invasiveness (8, 10). DDR2 is also upregulated in several pathological conditions, including hepatic fibrosis following injury, rheumatoid and osteoarthritis, and smooth muscle cell hyperplasia (8, 10-12).

1. Vogel, W.F. et al. (2006) Cell. Signal. 18:1108.
2. Karn, T. et al. (1993) Oncogene 8:3433.
3. Vogel, W. et al. (1997) Mol. Cell 1:13.
4. Leitinger, B. (2003) J. Biol. Chem. 278:16761.
5. Leitinger, B. and A.P.L Kwan (2006) Matrix Biol. 25:355.
6. Leitinger, B. et al. (2004) J. Mol. Biol. 344:993.
7. Mihai, C. et al. (2006) J. Mol. Biol. 361:864.
8. Olaso, E. et al. (2001) J. Clin. Invest. 108:1369.
9. Ikeda, K. et al. (2002) J. Biol. Chem. 277:19206.
10. Xu, L. et al. (2005) J. Biol. Chem. 280:548.
11. Wang, J. et al. (2002) J. Autoimmun. 19:161.
12. Ferri, N. et al. (2004) Am. J. Pathol. 164:1575.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDR1/DDR2 Antibody (MAB25382)(3)

Publications using MAB25382

• DS Corcoran, V Juskaite, Y Xu, F Görlitz, Y Alexandrov, C Dunsby, PMW French, B Leitinger DDR1 autophosphorylation is a result of aggregation into dense clusters Sci Rep, 2019;9(1):17104. 2019 [PMID: 31745115] (ICC, African Green Monkey)
• E Slocum, A Craig, A Villanueva, D Germain Parity predisposes breasts to the oncogenic action of PAPP-A and activation of the collagen receptor DDR2 Breast Cancer Res., 2019;21(1):56. 2019 [PMID: 31046834] (Western Blot, Human)
• ME Gonzalez, EE Martin, T Anwar, C Arellano-G, N Medhora, A Lama, YC Chen, KS Tanager, E Yoon, KM Kidwell, C Ge, RT Franceschi, CG Kleer Mesenchymal Stem Cell-Induced DDR2 Mediates Stromal-Breast Cancer Interactions and Metastasis Growth Cell Rep, 2017;18(5):1215-1228. 2017 [PMID: 28147276] (Western Blot, Human)

Bioinformatics

• Gene Symbol: DDR2
• Uniprot:
  • Q16832()