1336) for 10 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) Monoclonal Antibody (Catalog # MAB25382) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-DDR2 (Y740) at approximately 120-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> 1336) for 10 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) Monoclonal Antibody (Catalog # MAB25382) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-DDR2 (Y740) at approximately 120-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human DDR2 untreated (-) or treated (+) with 100 nM Calyculin A (Catalog # 1336) for 10 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) Monoclonal Antibody (Catalog # MAB25382) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-DDR2 (Y740) at approximately 120-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

1336) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-DDR2 (Y740) at approximately 130 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) Monoclonal Antibody (Catalog # MAB25382). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system." class="big_lightbox"> 1336) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-DDR2 (Y740) at approximately 130 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) Monoclonal Antibody (Catalog # MAB25382). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system." class="big_thumb" />