CYB5D1 Antibody

Images

 
Western Blot: CYB5D1 Antibody [NBP1-83529] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: CYB5D1 Antibody [NBP1-83529] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoli & the Golgi apparatus.
Immunohistochemistry-Paraffin: CYB5D1 Antibody [NBP1-83529] - Staining of human pancreas shows strong positivity in islet cells while exocrine cells were moderately positive.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CYB5D1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:EVAQHNRPEDLWVSYLGRVYDLTSLAQEYKGNLLLKPIVEVAGQDISHWFDPKTRDIRKHIDPLT
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYB5D1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CYB5D1 Protein (NBP1-83529PEP)

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (83%), Rat (85%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CYB5D1 Antibody

  • cytochrome b5 domain containing 1
  • cytochrome b5 domain-containing protein 1
  • FLJ32499

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CYB5D1 Antibody (NBP1-83529) (0)

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Secondary Antibodies

 

Isotype Controls

Additional CYB5D1 Products

Bioinformatics Tool for CYB5D1 Antibody (NBP1-83529)

Discover related pathways, diseases and genes to CYB5D1 Antibody (NBP1-83529). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for CYB5D1 Antibody (NBP1-83529)

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Bioinformatics

Gene Symbol CYB5D1