Cre Antibody [DyLight 405]

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Product Details

Summary
Reactivity Mu, CsSpecies Glossary
Applications WB, ICC/IF, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 405

Cre Antibody [DyLight 405] Summary

Immunogen
A synthetic peptide made to a portion of bacteriophage protein CRE (within amino acids 50-100). [UniProt# P06956]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This Cre antibody is useful for Immunoprecipitation, Western Blot, Immunohistochemistry-paraffin embedded sections, Immunohistochemistry-frozen sections, and Immunocytochemistry/Immunofluorescence (PMID: 21888903).

Reactivity Notes

Cre/lox Systems and Mouse. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Background

Cre recombinase or cyclization recombination enzyme is a bacteriophage-P1 enzyme belonging to large family of recombinases which are required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state. Cre is well known for facilitating a site-specific recombination between two 34 base pair loxP sites. If a DNA seqment is flanked by two loxP sites in the same orientation, Cre excises that seqment leaving a single loxP site in the remaining DNA molecule, and the excised DNA segment is subsequently degraded. Cre and lox comprises Cre/lox system which has been used extensively for multiple in vitro and in vivo applications including targeted gene deletions, gene-specific humanized animal models, as well as in plants and microorganisms stocks.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cre Antibody (NB100-56133V) (0)

There are no publications for Cre Antibody (NB100-56133V).
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cre Antibody (NB100-56133V). (Showing 1 - 1 of 1 FAQ).

  1. A customer is looking for a Cre Antibody, raised in either rabbit or goat, suitable for IHC-F. Would you know of a suitable Cre antibody that fits the above criteria and also been published for IHC-Fr? If known, can you also provide details of the publication?
    • With regards to your customer's inquiry on our Cre antibodies, we do have a wide range of antibodies that would be suitable to use in IHC for frozen sections. NB100-56134 for instance has been tested and validated for IHC-fry and there is one cited PubMed publication on it as well.

Secondary Antibodies

 

Isotype Controls

Additional Cre Products

Cre NB100-56133V

Blogs on Cre.

Understanding DNA Recombination with Cre-Lox
Cyclization recombination enzyme (Cre) is a member of the extensive family of recombinases and recognizes a 34 bp sequence motif from PI bacteriophage referred to as LoxP. The Cre enzyme works to cleanly excise an intervening DNA fragment that is flan...  Read full blog post.

Cre/Lox: The Genomic Utility Knife
Cre (Cyclization recombination enzyme) is a member of the large family of recombinases. Cre recognizes Lox site loxP, a 34 bp sequence motif from the PI bateriophage. If a DNA segment is flanked by two loxP sites in the same orientation, Cre neatly ex...  Read full blog post.

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