Cre Antibody [FITC]

Immunohistochemistry: Cre Antibody [FITC] [NB100-56133F] - Perfused mouse brain tissue. Dilution is 1:4000. This image was submitted via customer Review.

• Reactivity: Mu, Cs
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: FITC

Cre Antibody [FITC] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: A synthetic peptide made to a portion of bacteriophage protein CRE (within amino acids 50-100). [UniProt# P06956]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NB100-56133. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Specific to Cre/lox system (Bacteriophage). Shown to detect Cre protein in Cre-transfected human cells (HeLa) and Cre-transgenic mice. Mouse reactivity reported in scientific literature (PMID: 28194015).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Cre Antibody [FITC]

• Cyclization recombinase

Background

Cre recombinase or cyclization recombination enzyme is a bacteriophage-P1 enzyme belonging to large family of recombinases which are required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state. Cre is well known for facilitating a site-specific recombination between two 34 base pair loxP sites. If a DNA seqment is flanked by two loxP sites in the same orientation, Cre excises that seqment leaving a single loxP site in the remaining DNA molecule, and the excised DNA segment is subsequently degraded. Cre and lox comprises Cre/lox system which has been used extensively for multiple in vitro and in vivo applications including targeted gene deletions, gene-specific humanized animal models, as well as in plants and microorganisms stocks.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Cre Antibody (NB100-56133F). (Showing 1 - 1 of 1 FAQ).

1. A customer is looking for a Cre Antibody, raised in either rabbit or goat, suitable for IHC-F. Would you know of a suitable Cre antibody that fits the above criteria and also been published for IHC-Fr? If known, can you also provide details of the publication?
   • With regards to your customer's inquiry on our Cre antibodies, we do have a wide range of antibodies that would be suitable to use in IHC for frozen sections. NB100-56134 for instance has been tested and validated for IHC-fry and there is one cited PubMed publication on it as well.