![Western Blot: Connexin 35/36 [p Ser 276] Antibody [NBP3-11948] - Western blot of connexin 35/36 fusion proteins showing specific immunolabeling of the ~35 kDa connexin 35/36 phosphorylated at Ser276. The first lane (1) is PKA phosphorylated protein purified from the C-Terminal domain. The second lane (2) shows phosphospecificity indicated by no labeling in protein without PKA stimulation. The third lane (3) is protein purified from the intracellular loop, demonstrating that there is no cross-reactivity with the other major PKA phosphorylation site at Ser110.](http://images.novusbio.com/fullsize/Connexin-35-36-[p-Ser-276]-Antibody-Western-Blot-NBP3-11948-img0001.jpg "Western Blot: Connexin 35/36 [p Ser 276] Antibody [NBP3-11948] - Western blot of connexin 35/36 fusion proteins showing specific immunolabeling of the ~35 kDa connexin 35/36 phosphorylated at Ser276. The first lane (1) is PKA phosphorylated protein purified from the C-Terminal domain. The second lane (2) shows phosphospecificity indicated by no labeling in protein without PKA stimulation. The third lane (3) is protein purified from the intracellular loop, demonstrating that there is no cross-reactivity with the other major PKA phosphorylation site at Ser110.")
| Reactivity | Mu, Fi, RbSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide Free |
| Description | Novus Biologicals Rabbit Connexin 35/36 [p Ser276] Antibody - Azide Free (NBP3-11948) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser276 of Connexin 35/36 conjugated to KLH. (UniProt: O93328) |
| Modification | p Ser276 |
| Specificity | Specific for endogenous levels of the ~35 kDa connexin-35/36 protein phosphorylated at Ser276. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 35 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. |
| Buffer | 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol |
| Preservative | No Preservative |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Connexin 35/36 Antibody (NBP3-11948)Find related products by research area.
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