![Immunohistochemistry-Paraffin: CFAP54 Antibody [NBP1-90737] - Staining in human fallopian tube and placenta tissues using anti-CFAP54 antibody. Corresponding CFAP54 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/C12orf63-Antibody-Immunohistochemistry-Paraffin-NBP1-90737-img0008.jpg "Immunohistochemistry-Paraffin: CFAP54 Antibody [NBP1-90737] - Staining in human fallopian tube and placenta tissues using anti-CFAP54 antibody. Corresponding CFAP54 RNA-seq data are presented for the same tissues.")
| Reactivity | HuSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit CFAP54 Antibody - BSA Free (NBP1-90737) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: CQMKEYKLALLQCYGRYLQQFNTNFDENKVDVTQFKATFFPKGFKDKTAGLTFHALSGKNMCNYQLVCDSDENLKNKESVVQCLHILSSLRLIMQVA |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | C12ORF55 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | C12ORF55 |
![Immunocytochemistry/Immunofluorescence: CFAP54 Antibody [NBP1-90737] - Staining of human cell line U-2 OS shows localization to cytosol & microtubules.](http://images.novusbio.com/fullsize/C12orf63-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90737-img0005.jpg "Immunocytochemistry/Immunofluorescence: CFAP54 Antibody [NBP1-90737] - Staining of human cell line U-2 OS shows localization to cytosol & microtubules.")
![Immunohistochemistry-Paraffin: CFAP54 Antibody [NBP1-90737] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/CFAP54-Antibody-Immunohistochemistry-Paraffin-NBP1-90737-img0012.jpg "Immunohistochemistry-Paraffin: CFAP54 Antibody [NBP1-90737] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.")
![Immunohistochemistry-Paraffin: CFAP54 Antibody [NBP1-90737] - Staining of human fallopian tube shows moderate positivity in cilia of glandular cells.](http://images.novusbio.com/fullsize/C12orf63-Antibody-Immunohistochemistry-Paraffin-NBP1-90737-img0006.jpg "Immunohistochemistry-Paraffin: CFAP54 Antibody [NBP1-90737] - Staining of human fallopian tube shows moderate positivity in cilia of glandular cells.")
![Immunohistochemistry-Paraffin: CFAP54 Antibody [NBP1-90737] - Staining of human placenta shows low expression as expected.](http://images.novusbio.com/fullsize/C12orf63-Antibody-Immunohistochemistry-Paraffin-NBP1-90737-img0007.jpg "Immunohistochemistry-Paraffin: CFAP54 Antibody [NBP1-90737] - Staining of human placenta shows low expression as expected.")
![Immunohistochemistry-Paraffin: CFAP54 Antibody [NBP1-90737] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/CFAP54-Antibody-Immunohistochemistry-Paraffin-NBP1-90737-img0011.jpg "Immunohistochemistry-Paraffin: CFAP54 Antibody [NBP1-90737] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")