![Immunohistochemistry-Paraffin: CCDC75 Antibody [NBP1-93801] - Staining in human testis and pancreas tissues using anti-GPATCH11 antibody. Corresponding GPATCH11 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CCDC75-Antibody-Immunohistochemistry-Paraffin-NBP1-93801-img0010.jpg "Immunohistochemistry-Paraffin: CCDC75 Antibody [NBP1-93801] - Staining in human testis and pancreas tissues using anti-GPATCH11 antibody. Corresponding GPATCH11 RNA-seq data are presented for the same tissues.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit CCDC75 Antibody - BSA Free (NBP1-93801) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: KSGIGHEASLKRKAEEKLESYRKKIHMKNQAEEKAAEQFRMRLKNKQDEMKLEGDLRRSQRACQQLDVQKNIQVPREAWYWL |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GPATCH11 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | GPATCH11 |
![Western Blot: CCDC75 Antibody [NBP1-93801] - Analysis using Anti-GPATCH11 antibody NBP1-93801 (A) shows similar pattern to independent antibody NBP2-34044 (B).](http://images.novusbio.com/fullsize/CCDC75-Antibody-Western-Blot-NBP1-93801-img0013.jpg "Western Blot: CCDC75 Antibody [NBP1-93801] - Analysis using Anti-GPATCH11 antibody NBP1-93801 (A) shows similar pattern to independent antibody NBP2-34044 (B).")
![Immunocytochemistry/Immunofluorescence: CCDC75 Antibody [NBP1-93801] - Staining of human cell line U-251 MG shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CCDC75-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-93801-img0012.jpg "Immunocytochemistry/Immunofluorescence: CCDC75 Antibody [NBP1-93801] - Staining of human cell line U-251 MG shows localization to nucleus. Antibody staining is shown in green.")
![Western Blot: CCDC75 Antibody [NBP1-93801] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/CCDC75-Antibody-Western-Blot-NBP1-93801-img0006.jpg "Western Blot: CCDC75 Antibody [NBP1-93801] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunohistochemistry-Paraffin: CCDC75 Antibody [NBP1-93801] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/CCDC75-Antibody-Immunohistochemistry-Paraffin-NBP1-93801-img0008.jpg "Immunohistochemistry-Paraffin: CCDC75 Antibody [NBP1-93801] - Staining of human pancreas shows low expression as expected.")
![Immunohistochemistry-Paraffin: CCDC75 Antibody [NBP1-93801] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/CCDC75-Antibody-Immunohistochemistry-Paraffin-NBP1-93801-img0009.jpg "Immunohistochemistry-Paraffin: CCDC75 Antibody [NBP1-93801] - Staining of human testis shows high expression.")
![Immunohistochemistry-Paraffin: CCDC75 Antibody [NBP1-93801] -Staining of human skin shows moderate nuclear and cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/antibody/nbp1-93801_rabbit-polyclonal-ccdc75-antibody-immunohistochemistry-paraffin-262202495422..jpg "Immunohistochemistry-Paraffin: CCDC75 Antibody [NBP1-93801] -Staining of human skin shows moderate nuclear and cytoplasmic positivity in squamous epithelial cells.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")