![Western Blot: CCDC186 Antibody [NBP1-90440] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize2/CCDC186-Antibody-Western-Blot-NBP1-90440-img0010.jpg "Western Blot: CCDC186 Antibody [NBP1-90440] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC-P, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: LKRTFKEGMDELRTLRTKVKCLEDERLRTEDELSKYKEIINRQKAEIQNLLDKVKTADQLQEQLQRGKQEIENLKEEVESLNSLINDLQKDIEGSRKRESELLLFTERLTSKNAQLQSESNSLQSQFDKVSC |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CCDC186 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NBP1-90440 | Applications | Species |
|---|---|---|
| Topalidou I, Cattin-Ortola J, Hummer B et al. EIPR1 controls dense-core vesicle cargo retention and EARP complex localization in insulin-secreting cells Mol. Biol. Cell 2019-11-13 [PMID: 31721635] (ICC/IF, Rat) | ICC/IF | Rat |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CCDC186 |
![Immunocytochemistry/Immunofluorescence: CCDC186 Antibody [NBP1-90440] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/CCDC186-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90440-img0008.jpg "Immunocytochemistry/Immunofluorescence: CCDC186 Antibody [NBP1-90440] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: CCDC186 Antibody [NBP1-90440] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/CCDC186-Antibody-Immunohistochemistry-Paraffin-NBP1-90440-img0002.jpg "Immunohistochemistry-Paraffin: CCDC186 Antibody [NBP1-90440] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.")
![Western Blot: CCDC186 Antibody [NBP1-90440] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize2/CCDC186-Antibody-Western-Blot-NBP1-90440-img0009.jpg "Western Blot: CCDC186 Antibody [NBP1-90440] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")