Cadherin-23 Antibody

Western Blot: CDH23 Antibody [NBP1-20898] - Analysis of CDH23 in Human Amygdala lysate (35ug protein in RIPA buffer). Detected by chemiluminescence using this antibody at 1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Cadherin-23 Antibody Summary

• Immunogen: Peptide with sequence C-YNISLYENVTVGTS corresponding to internal region according to NP_071407.3, NP_443068.1.
• Epitope: C terminus
• Specificity: This antibody is expected to recognize both reported isoforms (NP_071407.3; NP_443068.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CDH23
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 60 kDa band observed in human brain (amygdala and frontal cortex lysates (calculated MW of 58.6 kDa band according to NP_443068.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cadherin-23 Antibody

• cadherin related 23
• cadherin-23
• cadherin-like 23
• cadherin-related 23
• cadherin-related family member 23
• CDHR23
• DKFZp434P2350
• FLJ00233
• FLJ36499
• KIAA1774
• KIAA1812
• MGC102761
• otocadherin
• USH1D

Background

This gene is a member of the cadherin superfamily, whose genes encode calcium dependent cell-cell adhesion glycoproteins. The encoded protein is a large, single-pass transmembrane protein composed of an extracellular domain containing 27 repeats that show significant homology to the cadherin ectodomain. Expressed in the neurosensory epithelium, the protein is thought to be involved in stereocilia organization and hair bundle formation. The gene is located in a region containing the human deafness loci DFNB12 and USH1D. Usher syndrome 1D and nonsyndromic autosomal recessive deafness DFNB12 are caused by allelic mutations of this cadherin-like gene. Two alternative splice variants have been identified that encode different isoforms. Additional variants have been observed but their full-length nature has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cadherin-23 Antibody (NBP1-20898)(1)

Publication using NBP1-20898

• Roux AF, Faugere V, Le Guedard S et al. Survey of the frequency of USH1 gene mutations in a cohort of Usher patients shows the importance of cadherin 23 and protocadherin 15 genes and establishes a detection rate of above 90%. J Med Genet 2006-09-01 [PMID: 16679490]

Bioinformatics

• Gene Symbol: CDH23
• Entrez:
  • 64072(Human)
• Uniprot:
  • Q9H251(Human)