BTNL8 Antibody (2187B) [Unconjugated]

Western blot shows lysates of Hek293T human embryonic kidney cell line either mock transfected or transfected with human BTNL8 and eGFP. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB9359) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for BTNL8 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HEK293 human embryonic kidney cell line transfected with human BTNL8 and eGFP was stained with either (A) Rabbit Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB9359) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by APC-conjugated Goat anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Simple Western lane view shows lysates of Hek293T human embryonic kidney cell line either mock transfected or transfected with with human BTNL8 and eGFP , loaded at 0.2 mg/mL. A specific band was detected for BTNL8 at approximately 66 kDa (as indicated) using 50 µg/mL of Rabbit Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB9359) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western lane view shows lysates of human small intestine tissue, loaded at 0.2 mg/mL. A specific band was detected for BTNL8 at approximately 60 kDa (as indicated) using 50 µg/mL of Rabbit Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB9359) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

• Reactivity: Hu
• Applications: WB, Simple Western, Flow
• Clone: 2187B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

BTNL8 Antibody (2187B) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human embryonic kidney cell line HEK-293-derived Human BTNL8
  Gln18-Lys238
  Accession # Q6UX41-1
• Specificity: Detects human BTNL8 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/5 x 10^6 cells
  • Simple Western 50 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BTNL8 Antibody (2187B) [Unconjugated]

• BTN9.2
• BTNL8
• butyrophilin-like 8
• butyrophilin-like protein 8
• UNQ702/PRO1347

Background

Butyrophilin-like 8 (BTNL8) is a member of the BTN/MOG Ig-superfamily and functions as a negative regulator of immune cell activation (1). Human BTNL8 is a 500 amino acid (aa) type I transmembrane glycoprotein that contains a signal peptide followed by an extracellular domain (ECD), a transmembrane region and a short cytoplasmic domain (2). The ECD of human BTNL8 shares 88% sequence identity with the ECD of mouse BTNL8. BTNL8 has two alternatively spliced forms: B7-like and BTN-like. Both isoforms of BTNL8 are expressed in a range of human tissues (3). The complete immunological function of BTNL molecules is only beginning to emerge. BTNL8 has been shown to be important in initiation of primary immune responses, suggesting a role in priming of naïve T lymphocytes (3). Down-regulation of BNTL8 mRNA levels has been associated with ulcerative colitis and colon cancer (4). BTNL8 are expressed in colon, lung, testis and neutrophils, and its expression is significantly decreased in ulcerative colitis, colonic tumors as compared to unaffected tissue (4). Soluble BTNL8-Fc fusion protein binds to resting, but not activated T cells. In vitro, BTNL8 co-stimulates T cell proliferation and cytokine production. In vivo injections of BTNL8-Fc significantly increases production of
Ag-specific IgG during the primary but not the secondary immune response (3).

1. Arnett, H.A. et al. (2007) J. Immunol. 178:1523.
2. Arnett, H.A. et al. (2009) Cytokine 46:370.
3. Chapoval, A.I. et al. (2013) Mol Immunol. 56:819.
4. Lebrero-Fernández C. et al. (2016) Immun Inflamm Dis. 4:191.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BTNL8 Antibody (MAB9359)(1)

Publication using MAB9359

• Toufic Mayassi, Kristin Ladell, Herman Gudjonson, James E. McLaren, Dustin G. Shaw, Mai T. Tran et al. Chronic Inflammation Permanently Reshapes Tissue-Resident Immunity in Celiac Disease Cell 2019-02-07 [PMID: 30739797]

Bioinformatics

• Uniprot:
  • Q6UX41-1()