Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/2032R) [CoraFluor™ 1]

• Reactivity: All-NA
• Applications: Func, IHC, CyTOF-ready
• Clone: BTN/2032R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/2032R) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Biotinylated sheep immunoglobulin
• Localization: Depends on the target
• Specificity: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This monoclonal antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • In-situ Hybridization
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/2032R) [CoraFluor™ 1]

• 58-85-5
• coenzyme R
• d-biotin
• Vitamin B7
• vitamin H

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.