Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/2032R) - Azide and BSA Free

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Immunohistochemistry-Paraffin: Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/2032R) - Azide and BSA Free [NBP2-61393] - Formalin-paraffin Tonsil stained with biotinylated Kappa Light Chain probe followed by anti-biotin ...read more

Product Details

Summary
Reactivity AllSpecies Glossary
Applications IHC, IHC-P, ISH, CyTOF-ready
Clone
BTN/2032R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/2032R) - Azide and BSA Free Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Biotinylated sheep immunoglobulin
Localization
Depends on the target
Specificity
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1 ug/ml
  • In-situ Hybridization
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Optimal dilution for a specific application should be determined.
Theoretical MW
244 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A purified

Notes

1.0 mg/ml of antibody purified by Protein A Column. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-61392).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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