beta-1,3-Glucuronyltransferase 3/B3GAT3 Antibody - Azide and BSA Free

Western Blot: beta-1,3-Glucuronyltransferase 3/B3GAT3 Antibody [H00026229-B01P] - Analysis of B3GAT3 expression in transfected 293T cell line by B3GAT3 polyclonal antibody. Lane 1: B3GAT3 transfected lysate(36.85 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

beta-1,3-Glucuronyltransferase 3/B3GAT3 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse beta-1,3-Glucuronyltransferase 3/B3GAT3 Antibody - Azide and BSA Free (H00026229-B01P) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-beta-1,3-Glucuronyltransferase 3/B3GAT3 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: B3GAT3 (NP_036332.2, 1 a.a. - 335 a.a.) full-length human protein. MKLKLKNVFLAYFLVSIAGLLYALVQLGQPCDCLPPLRAAAEQLRQKDLRISQLQAELRRPPPAPAQPPEPEALPTIYVVTPTYARLVQKAELVRLSQTLSLVPRLHWLLVEDAEGPTPLVSGLLAASGLLFTHLVVLTPKAQRLREGEPGWVHPRGVEQRNKALDWLRGRGGAVGGEKDPPPPGTQGVVYFADDDNTYSRELFEEMRWTRGVSVWPVGLVGGLRFEGPQVQDGRVVGFHTAWEPSRPFPVDMAGFAVALPLLLDKPNAQFDSTAPRGHLESSLLSHLVDPKDLEPRAANCTRVLVWHTRTEKPKMKQEEQLQRQGRGSDPAIEV
• Specificity: B3GAT3 - beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: B3GAT3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate in western blot. May also be used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for beta-1,3-Glucuronyltransferase 3/B3GAT3 Antibody - Azide and BSA Free

• B3GAT3
• beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I)
• Beta-1,3-glucuronyltransferase 3
• EC 2.4.1.135
• galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3
• GLCATI
• GlcAT-I
• GlcUAT-I
• Glucuronosyltransferase I
• Sqv-8-like protein
• UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase

Background

The protein encoded by this gene is a member of the glucuronyltransferase gene family, enzymes that exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product catalyzes the formation of the glycosaminoglycan-protein linkage by way of a glucuronyl transfer reaction in the final step of the biosynthesis of the linkage region of proteoglycans. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-1,3-Glucuronyltransferase 3/B3GAT3 Antibody (H00026229-B01P)(4)

Publications using H00026229-B01P

• Job F, Mizumoto S, Smith L et al. Functional validation of novel compound heterozygous variants in B3GAT3 resulting in severe osteopenia and fractures: expanding the disease phenotype. BMC Med Genet 2016-11-21 [PMID: 27871226]
• Budde BS, Mizumoto S, Kogawa R et al. Skeletal dysplasia in a consanguineous clan from the island of Nias/Indonesia is caused by a novel mutation in B3GAT3. Hum Genet. 2015-04-19 [PMID: 25893793]
• Baasanjav S, Al-Gazali L, Hashiguchi T et al. Faulty Initiation of Proteoglycan Synthesis Causes Cardiac and Joint Defects. Am J Hum Genet. 2011-07-15 [PMID: 21763480]
• Hiyama A, Gajghate S, Sakai D et al. Activation of TonEBP by Calcium Controls {beta}1,3-Glucuronosyltransferase-I Expression, a Key Regulator of Glycosaminoglycan Synthesis in Cells of the Intervertebral Disc. J Biol Chem;284(15):9824-9834. 2009-01-01 [PMID: 19147493]

Bioinformatics

• Gene Symbol: B3GAT3
• Entrez:
  • 26229(Human)
• Uniprot:
  • NP_036332.2(Human)