Baboon Antibody

Images

 
Western Blot: Baboon Antibody [NB300-665] - Lane 1 and Land 2: Cells expressing Baboon A and Lane 3: mock transfected cells

Product Details

Summary
Reactivity DrSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.75 mg/ml

Order Details

Baboon Antibody Summary

Immunogen
Synthetic peptide: QPDPSIEEMKKVVCIEKCRPN, corresponding to amino acids 552 to 572 of Baboon.The was generated against a peptide sequence near the C terminal end of the protein.The peptides was amidated for conjugation.
Specificity
The is unique to Baboon protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:200
  • Immunoprecipitation 1:200

Reactivity Notes

Fruit fly.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol
Preservative
0.02% Sodium Azide
Concentration
0.75 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Baboon Antibody

  • Activin A receptor 45A
  • ATR 1
  • Atr 45A
  • Atr I
  • ATR1
  • Atr45A
  • AtrI
  • Bab
  • Babo
  • CG8224
  • G protein coupled receptor kinase 3
  • Gprk 3
  • Gprk3
  • l(2)k16912
  • RE55648p
  • STK E
  • STKE

Background

Transmembrane receptor Patched regulates several developmental processes in both invertebrate and vertebrates. In higher vertebrates the patched (Ptc) also acts as tumor suppressor. The patched receptor signaling involves negatively regulating a GPCR, smoothened. The binding Hedgehog ligand to Ptc, removes the negative interaction of Ptc and allows the signaling by smoothened. It has been shown that Ptc D. melanogasater head development. During head morphogenesis in D. melanogaster Ptc positively interacts with smoothened, which leads to stimulation of Activin type 1 receptor Baboon and activation of cell proliferation in the eye-antennal disc (3). The expression of activated Baboon in the Ptc domain in Ptc mutant background completely abolish the head developmental defects. Baboon is a 601 amino acid long (Mr. Wt. 73 kDa) protein with a single transmembrane domain. Novus International Inc. has produced epitope specific anti-Baboon antibodies in rabbit. The Anti-Baboon-selective antibodies were generated against a peptide sequence near the C-terminal end of the protein that are unique to Baboon protein. The Baboon-selective antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded epitope-specific antibodies. The Baboon antibodies label a 73-74 kDa protein in D. melanogaster extracts. Anti-Baboon-selective antibodies are also available in affinity-purified form for confocal, Western blotting and immunocytochemical analyses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Baboon Products

Baboon NB300-665

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