Baboon Antibody

Western Blot: Baboon Antibody [NB300-664] - Lane 1 and 2 : Cells expressing Baboon A. Lane 3: mock trasfected cells

• Reactivity: Dr
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Baboon Antibody Summary

• Immunogen: Synthetic peptide: CNKNENMKRIFETDYVPHRLTS, corresponding to N terminal amino acids 200-221 of Drosophilia melanogaster Baboon. The peptide was amidated for conjugation.
• Specificity: The is unique to Baboon protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunohistochemistry
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
• Application Notes: Immunocytochemistry, Immunoprecipitation, Western Blot Detects a band of approximately 73 kDa. Optimal dilutions and concentrations should be determined by the end user.

Reactivity Notes

Fruit fly.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Baboon Antibody

• Activin A receptor 45A
• ATR 1
• Atr 45A
• Atr I
• ATR1
• Atr45A
• AtrI
• Bab
• Babo
• CG8224
• G protein coupled receptor kinase 3
• Gprk 3
• Gprk3
• l(2)k16912
• RE55648p
• STK E
• STKE

Background

Transmembrane receptor Patched regulates several developmental processes in both invertebrate and vertebrates. In higher vertebrates the patched (Ptc) also acts as tumor suppressor. The patched receptor signaling involves negatively regulating a GPCR, smoothened. The binding Hedgehog ligand to Ptc, removes the negative interaction of Ptc and allows the signaling by smoothened. It has been shown that Ptc D. melanogasater head development. During head morphogenesis in D. melanogaster Ptc positively interacts with smoothened, which leads to stimulation of Activin type 1 receptor Baboon and activation of cell proliferation in the eye-antennal disc (3). The expression of activated Baboon in the Ptc domain in Ptc mutant background completely abolish the head developmental defects. Baboon is a 601 amino acid long (Mr. Wt. 73 kDa) protein with a single transmembrane domain. FabGennix International Inc. has produced epitope specific anti-Baboon antibodies in rabbit. The Anti-Baboon-selective antibodies were generated against a peptide sequence near the C-terminal end of the protein that are unique to Baboon protein. The Baboon-selective antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded epitope-specific antibodies. The Baboon antibodies label a 73-74 kDa protein in D. melanogaster extracts. Anti-Baboon-selective antibodies are also available in affinity-purified form for confocal, Western blotting and immunocytochemical analyses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.