B4GALT3 Antibody

Western Blot: B4GALT3 Antibody [NBP3-03998] - Analysis of extracts of HeLa cells, using B4GALT3 antibody . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Product Discontinued

B4GALT3 Antibody Summary

• Immunogen: A synthetic peptide of human B4GALT3
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: B4GALT3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 50% glycerol, pH7.3.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for B4GALT3 Antibody

• b4Gal-T3
• beta-1,4-galactosyltransferase 3
• Beta-1,4-GalTase 3
• beta4Gal-T3
• beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase 3
• EC 2.4.1.-
• UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3
• UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3
• UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3

Background

Several oligosaccharide structures and protein glycoconjugate types are found in nature. Homologous glycosyltransferase (GT) gene families catalyze the formation of glycosidic linkages. The beta-1,3 galactosyltransferase(beta3GalT) gene family encodes a set of type II transmembrane glycoproteins that are catalytically diverse and use different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine ) to catalyze the addition of an activated monosaccharide to a terminal lactose. The protein coding sequences for beta-1,3-Gal-T genes comprise a single exon and are distantly related to the Drosophila Brainiac gene. The beta-1,4-galactosyltransferase (beta4GalT) gene family encodes type II membrane-bound glycoproteins that show exclusive specificity for the donor substrate, UDP-galactose. beta-1,4Gal-T genes transfer galactose in a beta-1,4 linkage to similar acceptor sugars; each gene has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: B4GALT3